scholarly journals Screening Red Raspberry for Cold Hardiness in Vitro

HortScience ◽  
1993 ◽  
Vol 28 (7) ◽  
pp. 740-741 ◽  
Author(s):  
Annette M. Zatylny ◽  
J.T.A. Proctor ◽  
J.A. Sullivan
Keyword(s):  
Low Temperatures ◽  
Cold Hardiness ◽  
Screening Method ◽  
Temperature Acclimation ◽  
Rubus Idaeus ◽  
Red Raspberry ◽  
Visual Rating ◽  
In Vitro Shoots ◽  
Cold Hardy

Two selections and two cultivars of red raspberry (Rubus idaeus L.) were evaluated for cold hardiness in vitro. Tissue-cultured shoots were exposed to temperatures from 0 to –18C and samples were removed at 2C intervals. Injury was assessed by a visual rating of tissue browning after freezing. Only shoots subjected to step-wise acclimation at low temperatures before freezing revealed significant differences among the four types in the lowest shoot survival temperature. Acclimation treatments increased the lowest survival temperatures of in vitro shoots by a mean of 3.1C. The hardiness obtained from this screening method agreed with that of winter survival in the field. Ranking, from the most to least cold hardy, was `Boyne', Gu 72, Gu 63, and `Comox'.

scholarly journals Effect of Elevated Medium Sucrose Levels on Cold Hardiness of `Festival' Red Raspberry in Vitro

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 850D-850
Author(s):  
Pauliina Palonen ◽  
Danielle Donnelly ◽  
Deborah Buszard
Keyword(s):  
Cold Hardiness ◽  
Sugar Content ◽  
Rubus Idaeus ◽  
Red Raspberry ◽  
Tissue Water ◽  
Osmotic Concentration ◽  
Visual Rating ◽  
The Media ◽  
Different Levels

Low tissue-water content and increased osmotic concentration of cell sap are associated with frost resistance. Changes in total osmotic concentration of cell sap are due mainly to changes in concentration of sugars. Generally, sugar content increases with hardening and decreases with dehardening. This study examined the effect of elevated sucrose levels (3% to 15%) in the medium on the cold hardiness of `Festival' red raspberry (Rubus idaeus L.) shoots in vitro. To determine whether expected hardening is caused by elevated sucrose levels or by osmotic stress, different levels of mannitol in the media have been tested. After growing raspberry shoots on media with different levels of sucrose and mannitol for 2 weeks, shoot moisture content (percent) was determined. Cold hardiness of the shoots was determined by using differential thermal analysis or artificially freezing the shoots and assessing the survival by regrowth test and visual rating.

scholarly journals Relating Freeze-induced Electrolyte Leakage Measurements to Lethal Temperature in Red Raspberry

2000 ◽  
Vol 125 (4) ◽  
pp. 429-435 ◽  
Author(s):  
Leena Lindén ◽  
Pauliina Palonen ◽  
Mikael Lindén
Keyword(s):  
Electrolyte Leakage ◽  
Cold Hardiness ◽  
Rubus Idaeus ◽  
Data Sets ◽  
Red Raspberry ◽  
Lethal Temperature ◽  
Gompertz Function ◽  
Visual Rating ◽  
Richards Function ◽  
Response Percentage

Seasonal cold hardiness of red raspberry (Rubus idaeus L.) canes was measured by freeze-induced electrolyte leakage test and visual rating of injury. Leakage data were transformed to percentage-adjusted injury values and related to lethal temperature by graphical interpolation and by the midpoint (T50) and inflection point (Tmax) estimates derived from three sigmoid (the logistic, Richards, and Gompertz) functions. Tmax estimates produced by Richards and Gompertz functions were corrected further using two different procedures. The 10 leakage-based hardiness indices, thus derived, were compared to lethal-temperature estimates based on visual rating. Graphical interpolation and Tmax of the logistic or T50 of the Gompertz function yielded lethal-temperature estimates closest to those obtained visually. Also, Tmax values of the Gompertz function were well correlated with visual hardiness indices. The Richards function yielded hardiness estimates deviating largely from visual rating. In addition, the Richards function displayed a considerable lack of fit in several data sets. The Gompertz function was preferred to the logistic one as it allows for asymmetry in leakage response. Percentage-adjusted injury data transformation facilitated curve-fitting and enabled calculation of T50 estimates.

Flower cold hardiness: a potential determinant of the flowering sequence exhibited by bog ericads

1979 ◽  
Vol 57 (9) ◽  
pp. 997-999 ◽  
Author(s):  
R. J. Reader
Keyword(s):  
Low Temperatures ◽  
Cold Hardiness ◽  
Vaccinium Macrocarpon ◽  
Southern Ontario ◽  
Air Temperatures ◽  
Insect Pollinators ◽  
Cold Hardy ◽  
Vaccinium Myrtilloides ◽  
Potential Determinant ◽  
Ledum Groenlandicum

In laboratory freezing trials, cold hardiness of six types of bog ericad flowers differed significantly (i.e., Chamaedaphne calyculata > Andromeda glaucophylla > Kalmia polifolia > Vaccinium myrtilloides > Ledum groenlandicum > Vaccinium macrocarpon) at air temperatures between −4 and −10 °C but not at temperatures above −2 °C. At the Luther Marsh bog in southern Ontario, low temperatures (−3 to −7 °C) would select against May flowering by the least cold hardy ericads. Availability of pollinators, on the other hand, would encourage May flowering by the most cold hardy species. Presumably, competition for insect pollinators has promoted the diversification of bog ericad flowering peaks, while air temperature, in conjunction with flower cold hardiness, determined the order in which flowering peaks were reached.

scholarly journals Super Cooling Point Phenotypes and Cold Resistance in Hyles euphorbiae Hawk Moths from Different Climate Zones

Diversity ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 207
Author(s):  
Hana Daneck ◽  
Matthias Benjamin Barth ◽  
Martin Geck ◽  
Anna K. Hundsdoerfer
Keyword(s):  
Environmental Conditions ◽  
Cold Hardiness ◽  
Cold Treatment ◽  
Underlying Mechanism ◽  
Frost Tolerance ◽  
Cold Sensitivity ◽  
Cold Hardy ◽  
Hyles Euphorbiae

The spurge hawkmoth Hyles euphorbiae L. (Sphingidae) comprises a remarkable species complex with still not fully resolved taxonomy. Its extensive natural distribution range covers diverse climatic zones. This predestinates particular populations to cope with different local seasonally unfavorable environmental conditions. The ability of the pupae to overcome outer frosty conditions is well known. However, the differences between two main ecotypes (‘euphorbiae’ and ‘tithymali’) in terms of the inherent degree of frost tolerance, its corresponding survival strategy, and underlying mechanism have not been studied in detail so far. The main aim of our study was to test the phenotypic exhibition of pupae (as the relevant life cycle stadia to outlast unfavorable conditions) in response to combined effects of exogenous stimuli, such as daylight length and cooling regime. Namely, we tested the turnout of subitan (with fast development, unadapted to unfavorable conditions) or diapause (paused development, adapted to unfavorable external influences and increased resistance) pupae under different conditions, as well as their mortality, and we measured the super cooling point (SCP) of whole pupae (in vivo) and pupal hemolymph (in vitro) as phenotypic indicators of cold acclimation. Our results show higher cold sensitivity in ‘tithymali’ populations, exhibiting rather opportunistic and short-termed cold hardiness, while ‘euphorbiae’ produces a phenotype of seasonal cold-hardy diapause pupae under a combined effect of short daylight length and continuous cold treatment. Further differences include the variability in duration and mortality of diapause pupae. This suggests different pre-adaptations to seasonal environmental conditions in each ecotype and may indicate a state of incipient speciation within the H. euphorbiae complex.

scholarly journals Breaking Dormancy in Red Raspberry with Hot Water Treatment and its Effects on Cold Hardiness

2006 ◽  
Vol 131 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Pauliina Palonen ◽  
Leena Lindén
Keyword(s):  
Water Treatment ◽  
Cold Hardiness ◽  
Treatment Temperature ◽  
Rubus Idaeus ◽  
Hot Water ◽  
Bud Dormancy ◽  
Hot Water Treatment ◽  
Red Raspberry ◽  
Breaking Dormancy ◽  
Water Treatments

`Maurin Makea', `Muskoka', ` Ottawa', and `Preussen' red raspberry (Rubus idaeus L.) canes were collected from the field and subjected to different hot water treatments (20, 35, 40, 45, and 50 °C) to determine if endodormancy could be removed by a near lethal stress. Estimation of days for 50% budbreak (DD50) was found useful for describing the state of bud dormancy in the samples. Bud dormancy was broken in `Ottawa' by immersing the canes in 45 °C water for 2 hours, in `Maurin Makea' by treating the canes in 40 °C water, and in `Preussen' by both 40 and 45 °C treatments. The influence of this treatment on dormancy and cold hardiness at different times of the winter was further examined using `Ottawa' raspberry. The treatment removed bud dormancy most effectively in October, when the samples were in deepest dormancy. A slight effect was observed in November, but no effect in January. During ecodormancy in February the treatment delayed budbreak. Hot water treatment reduced cold hardiness of `Ottawa' canes by 8 to 15 °C, and that of buds by 9 to 13 °C during both endo- and ecodormancy. Based on the capacity of buds and canes to reacclimate, recovery from the stress treatment was possible at temperatures ≥4 °C. Loss of cold hardiness was caused by high treatment temperature itself and was not related to breaking of dormancy in samples. This finding suggests that dormancy and cold hardiness are physiologically unconnected in raspberry.

Cold-Hardiness, Habitat and Winter Survival of Some Orchard Arthropods in Nova Scotia

1964 ◽  
Vol 96 (4) ◽  
pp. 617-625 ◽  
Author(s):  
A. W. MacPhee
Keyword(s):  
Nova Scotia ◽  
Air Temperature ◽  
Low Temperatures ◽  
Cold Hardiness ◽  
Winter Survival ◽  
Kings County ◽  
Winter Conditions ◽  
Cold Hardy

AbstractIn Kings County, Nova Scotia, low temperatures in the coldest nights of winter can differ by as much as 10°F. from one area to another. This has an important bearing on winter survival of some arthropods. Overwintering sites of orchard arthropods range from exposed situations which remain at air temperature to well protected ones on the ground where temperatures rarely go below 20°F. The cold-hardiness of each of 24 species of arthropods was measured: seven were sufficiently cold-hardy to survive any winter conditions in Nova Scotia, five were less cold-hardy but overwinter in well protected sites and twelve had marginal cold-hardiness, their mortality varying with the winter and the locality.

In vitro hardening of red raspberry through CO2 enrichment and relative humidity reduction on sugar-free medium

1993 ◽  
Vol 73 (4) ◽  
pp. 1105-1113 ◽  
Author(s):  
Ribo Deng ◽  
Danielle J. Donnelly
Keyword(s):  
Relative Humidity ◽  
Co2 Enrichment ◽  
Dry Weight ◽  
Early Growth ◽  
Rubus Idaeus ◽  
Ex Vitro ◽  
Red Raspberry ◽  
Plantlet Growth ◽  
Murashige Skoog

Micropropagated shoots of red raspberry (Rubus idaeus L. ’Comet’) were rooted on modified Murashige-Skoog medium lacking sucrose, in specially constructed plexiglass chambers, under ambient (340 ± 20 ppm) or enriched (1500 ± 50 ppm) CO2 and ambient (ca. 100%) or reduced (90 ± 5%) relative humidity. Cultured plantlets were evaluated for their survival, rooting and relative vigor, leaf and root number, stem and root length, total leaf area, total fresh and dry weight, gas exchange rate, and stomatal features, prior to transplantation to soil and at intervals for 6 wk ex vitro. In vitro CO2 enrichment promoted plantlet growth, rooting and both the survival and early growth of transplants. CO2 enrichment increased stomatal aperture of plantlet leaves but did not apparently increase water stress at transplantation. Reduced in vitro RH did not affect plantlet growth but decreased stomatal apertures and stomatal index on leaves of cultured plantlets and promoted both the survival and early growth of transplants. In vitro CO2 and RH levels did not affect the photosynthetic rate of either plantlets or transplants. Only the stomata on leaves of plantlets from the ambient CO2 and reduced RH treatment were functional. Normal stomatal function was not observed in persistent leaves of transplants from the other treatments, even 2 wk after transplantation. In vitro CO2 enrichment acted synergistically with RH reduction in improving growth of plantlets both in vitro and ex vitro. Hardened red raspberry plantlets obtained through CO2 enrichment and RH reduction survived direct transfer to ambient greenhouse conditions without the necessity for specialized ex vitro acclimatization treatment. Key words: Acclimatization, growth analysis, photosynthesis, Rubus idaeus L., stomata, tissue culture

scholarly journals Dormancy, Cold Hardiness, Dehardening, and Rehardening in Selected Red Raspberry Cultivars

1999 ◽  
Vol 124 (4) ◽  
pp. 341-346 ◽  
Author(s):  
Pauliina Palonen ◽  
Leena Lindén
Keyword(s):  
Cold Hardiness ◽  
The State ◽  
Rubus Idaeus ◽  
Cultivar Differences ◽  
Dormancy Release ◽  
Red Raspberry ◽  
Flower Buds

Canes and flower buds of selected red raspberry cultivars (Rubus idaeus L. `Maurin Makea', `Muskoka', and `Ottawa') were sampled from a field (latitude, 61 °20'N; longitude, 24 °13'E) at 1-month intervals during Winter 1996-97 to study the interaction of dormancy and cold hardiness, hardiness retention, and rehardening capacity. One set of canes was subjected to dehardening (3 days) and two sets to dehardening + rehardening (3 and 7 days) treatments before cold hardiness determination. Maximum midwinter hardiness occurred in January, after breaking of endodormancy. Cold hardiness of canes and buds reached -28.6 to -37.2 °C and -24.2 to -31.6 °C, respectively. Throughout the winter, raspberry canes were hardier than buds. Endodormancy had a greater influence on dehardening and rehardening in buds than in canes, and cultivars differed in their response. Dehardening of `Maurin Makea' canes and buds, and `Muskoka' buds was slightly enhanced by breaking of dormancy, whereas dehardening in `Ottawa' was not affected by dormancy. Raspberry canes and buds could reharden even after dormancy release. Rehardening capacity was affected by the state of dormancy only in `Maurin Makea' buds. Changes in dormancy status failed to explain cultivar differences regarding dehardening and the capacity to reharden suggesting other factors may be involved.

THE EFFECT OF LONG EXPOSURE TO LOW TEMPERATURES ON THE COLD HARDINESS OF SPROUTING WHEAT IN THE DARK

1985 ◽  
Vol 65 (4) ◽  
pp. 893-900 ◽  
Author(s):  
D. W. A. ROBERTS
Keyword(s):  
Triticum Aestivum ◽  
Low Temperatures ◽  
Cold Hardiness ◽  
Triticum Aestivum L ◽  
Late Winter ◽  
Initial Increase ◽  
Winter Mortality ◽  
Canadian Prairies ◽  
Northern Regions ◽  
Cold Hardy

Nine cultivars of common wheat (Triticum aestivum L.) ranging from very cold hardy to tender were sprouted in vermiculite at 0.5–1.0 °C for 7 wk in the dark and then placed at 0.5 °C, −2.5 °C, −5 °C, −7.5 °C, or −10 °C for up to 20 wk. Plants held at 0.5 °C progressively lost hardiness. Little change occurred in the hardiness of plants moved to −2.5 °C. There was apparently a small initial increase in hardiness after transfer to −5 °C or −7.5 °C followed by a decline in hardiness. Plants transferred to −10 °C lost hardiness progressively after transfer. These results suggest that part of the reason for late-winter mortality of winter wheats in northern regions of the Canadian prairies is damage from long exposures to temperatures only slightly lower than −5 °C. This damage is manifested by higher LT50 values or lower cold hardiness in late winter and early spring.Key words: Triticum aestivum L., cold hardiness, winter survival

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