scholarly journals Effect of Oak Tree Sawdust Fermentation Period on Peanut Seed Germination, Seedling Biomass, and Morphology

Horticulturae ◽  
2021 ◽  
Vol 7 (7) ◽  
pp. 182
Author(s):  
Junsik Ahn ◽  
Soyeon Oh ◽  
Yang Joo Kang ◽  
KiBum Kim ◽  
Sung-Kwon Moon ◽  
...  
Keyword(s):  
Microbial Community ◽  
Seed Germination ◽  
Germination Rate ◽  
Peanut Seed ◽  
Arachis Hypogaea L ◽  
Vigor Index ◽  
Seedling Biomass ◽  
Oak Tree ◽  
Fermentation Period ◽  
Seedling Characteristics

Peanut (Arachis hypogaea L.) seeds were germinated to investigate the effect of the fermentation period of oak tree sawdust on germination viability and seedling characteristics. Its germination rate, seedling weight, length, and total vigor index were assessed. The seeds were sown in oak tree sawdust fermented for 0, 30, 45, and 60 days. The germination rates of the seeds in fermented sawdust were significantly different. The seeds in the 45-day fermented sawdust produced the heaviest biomass weight (4.6 g) with the longest true leaf (1.7 cm) and hypocotyl (3.4 cm) resulting in the highest total vigor index (925.8). In contrast, seeds in 0-day fermented sawdust had the lowest total vigor index (18.3). Microbiome analysis showed that the microbial community in the sawdust changed as the fermentation progressed, indicating that the microbial community seems to affect seed germination physiology. Taken together, 45-day fermented sawdust is recommended for optimal peanut seed germination and seedling growth.

scholarly journals Expression of MYB transcription factor gene ZmMYB59 affects seed germination in Nicotiana tabacum and Oryza sativa

2020 ◽  
Author(s):  
Kaihui Zhai ◽  
Guangwu Zhao ◽  
Hongye Jiang ◽  
Caixia Sun ◽  
Jingyu Ren
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Seed Germination ◽  
Transgenic Tobacco ◽  
Germination Rate ◽  
Myb Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Myb Transcription Factors ◽  
Vigor Index

Abstract Background MYB transcription factors are involved in many biological processes, including metabolism, development and responses to biotic and abiotic stresses. In our previous work, a new MYB transcription factor gene, ZmMYB59 was induced by deep sowing and down-regulated during maize seed germination via Real-Time PCR. However, there are few reports on seed germination regulated by MYB proteins and the functions of ZmMYB59 remain unknown. Results In this study, to examine its functions, Agrobacterium -mediated transformation was exploited to generate ZmMYB59 transgenic tobacco and rice. In T 2 generation transgenic tobacco, germination rate, germination index, vigor index and hypocotyl length were significantly decreased by 25.0~50.9%, 34.5~54.4%, 57.5~88.3% and 21.9~31.2% compared to wild-type (WT) lines. In T 2 generation transgenic rice, germination rate, germination index, vigor index and mesocotyl length were notably reduced by 39.1~53.8%, 51.4~71.4%, 52.5~74.0% and 28.3~41.5%, respectively. On this basis, relative physiological indicators were determined. The activities of catalase, peroxidase, superoxide dismutase, ascorbate peroxidase and proline content of transgenic lines were significantly lower than those of WT, suggesting that ZmMYB59 reduced their antioxidant capacity. As well, ZmMYB59 expression extremely inhibited the synthesis of gibberellin A1 (GA 1 ) and cytokinin (CTK), and promoted the synthesis of abscisic acid (ABA) concurrently, which implied that seed germination was repressed by ZmMYB59 in hormone levels. Furthermore, cell length and cell number of hypocotyl/mesocotyl in transgenic plants were notably decreased. Conclusions Taken together, it proposed that ZmMYB59 plays a negative regulation during seed germination in tobacco and rice, which also contributes to illuminate the molecular mechanisms regulated by MYB transcription factors.

scholarly journals PENGGUNAAN Trichoderma Koningii PADA PERKECAMBAHAN SIRSAK (Annona muricata linn)

2019 ◽  
Vol 15 (3) ◽  
pp. 563
Author(s):  
Erida Derita Dalame ◽  
Bertje Richard Albert Sumayku ◽  
Jeany Polii - Mandang
Keyword(s):  
Seed Germination ◽  
Plant Protection ◽  
Germination Rate ◽  
Soaking Time ◽  
The North ◽  
North Sulawesi ◽  
Trichoderma Koningii ◽  
Randomized Design ◽  
Vigor Index ◽  
Two Factors

This study aims to determine the dose of Trichoderma koningii which can induce soursop germination; know the best soaking time that can affect the induction of soursop germination; to know the interaction between soaking time and Trichoderma koningii dose on soursop germination. This research was conducted in April - June 2019 at the Green House of Plant Sciences, Faculty of Agriculture, Sam Ratulangi University, Manado. The material used in this study was soursop seed obtained from Lota Village, Pineleng Tomohon Subdistrict, Trichoderma koningii with 106 solids obtained from the North Sulawesi Province Plant Protection Center Collection, Aquadest, 70% Alcohol, 80% Acetone, sandalready sifter and sterilized. This research was arranged in factorial with a Completely Randomized Design (CRD) consisting of two factors, namely 5 treatments of Trichoderma koningii dose and 4 immersion treatments repeated three times (3x) so that a total of 60 treatment units. Each treatment unituses 10 seeds. The results of this study indicated that the dose of Trichoderma koninggi 200 g without soaking can increase the germination power by 86.67% and the vigor index by 32.96%; Immersion with Koninggi Trichoderma suspension has not been able to induce soursop seed germination rate; Theinteraction between Trichoderma koningii dose and soaking time did not affect the speed of soursop seed germination.*eprm*

scholarly journals SETTINGS OF TEMPERATURE AND TIME SAVING ON SEED GERMINATION OF Magnolia champaca (L.) Baill. ex Pierre

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Odetta Maudy Nuradinda ◽  
Sri Pertiwi Estuningsih ◽  
Harmida Harmida
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Temperature Treatment ◽  
Seed Vigor ◽  
Control Treatment ◽  
Time Saving ◽  
Randomized Design ◽  
Vigor Index ◽  
Germination Vigor ◽  
Physiology Laboratory

Setting storage space temperature and time saving becomes an important application in supporting the development of plantations to provide seeds as needed and sustainable. The purpose of this research is to delay the rapid of seed germination in Magnolia champaca by assessing the effect of various suspend temperatures and times saving on seed germination of Magnolia camphaca . The research was conducted in Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Sriwijaya University in February until June 2016. The method was used completely randomized design pattern Factorial by 3 factors with 12 treatments and 3 replications with the temperature: temperature ± 31°C, temperature ± 25 °C, temperature ± 18 °C, temperature ± 4 °C and the retention of time 0 week, 2 weeks , 4 weeks and 6 weeks. The parameters include the observation of germination rate, germination, vigor index and sprout morphology. The results of the research the treatment of interaction of temperature and time saving significantly affected on germination rate, germination and seed vigor index of Magnolia champaca. Germination speed, germination and vigor index were both produced by the treatment of storage at a temperature of ± 18 °C and a temperature of ± 25 °C at all times to keep (2 weeks, 4 weeks, 6 weeks) compared to control treatment. A normal sprout produced in temperature treatment ± 18 °C and ± 25 °C temperature ranges between 90-93%. An abnormal sprout produced in control treatment (0 week) by 25%. Measurement of moisture content and physiological maturity level of Magnolia champaca seed needs to be done before it is stored and germinated to test the viability of seeds of sprouts.

scholarly journals The Effect of Invigoration Using Polyethylene Glycol And Ultra Fine Bubble on Improving of Sengon Seeds (Falcataria Moluccana Miq.) Quality After Two Years Storage

Jurnal Wasian ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 133-143
Author(s):  
Iskandar Siregar ◽  
◽  
Riki Ramdhani ◽  
Evayusvita Rustam ◽  
Dede Sudrajat ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Seed Viability ◽  
Germination Capacity ◽  
Peg 6000 ◽  
Vigor Index ◽  
Fine Bubble ◽  
Initial Seed ◽  
Environmental Air

Utilization of seeds storage results in decreased plant productivity. The increase can be used by treating seeds before planting through the technique of invigoration. This purpose of the study is to assess the effectiveness of invigoration methods using polyethylene glycol (PEG 6000) and ultrafine bubbles (UFB) to improve viability and vigor of sengon seeds (Falcataria moluccana). Seed agieng using 96 % ethanol was carried out to obtain the diversity of seedlot viability as the materials for testing the effectiveness of invigoration treatments. A complete random design was used to test the effectiveness of 5 invigoration treatments, i.e., seed without invigoration treatment, soaking in PEG 6000 -0.8 Mpa, soaking in PEG 6000 -1.2 Mpa, soaking in UFB water injected by environmental air, and soaking in UFB water injected by oxygen 99 %, with soaking time is 24 hours for each treatment. Seed agieng resulted three classes of seed viability, i.e. 62 % (initial seed), 83 % (seed agieng for 30 minutes) and 57 % (seed agieng for 60 minutes). In the condition of seed germination before treatment (DBA) 57 % and DBA 62%, invigoration treatments were significantly affected on seed germination capacity, but not significantly different in DBA 83 %. The soaking treatment of UFB injected by oxigen 99 % was able to improve the germination parameters (germination capacity, germination rate, and vigor index) on the sengon seeds with DBA 57 %. For seeds with DBA 62 %, the soaking treatment in UFB injected by environmental air was provided the best germination capacity, T50, and vigor index. The treatment of UFB injected by oxygen 99 % was more effective to improve the seed with very low viability and vigor (DBA 57 %). In general, improving of seed viability and vigor is more effective by using UFB than PEG 6000.

scholarly journals Effect of medicinal plant extracts on seed germination and seed associated mycoflora of wheat (Triticum aestivum L.)

2019 ◽  
Vol 27 ◽  
pp. 23-30
Author(s):  
MF Mahal ◽  
MS Akter
Keyword(s):  
Seed Germination ◽  
Plant Extracts ◽  
Germination Rate ◽  
Trichoderma Viride ◽  
Bipolaris Sorokiniana ◽  
Tagetes Patula ◽  
Wheat Varieties ◽  
Vigor Index ◽  
Medicinal Plant Extracts

The experiment was carried to evaluate the efficacy of five different plant extracts i.e. Adhatida vasica, Tagetes patula, Vinca rosea, Lowsonia alba and Andrographis paniculuta on seed germination, vigor index and seed borne and seed associated mycoflora of wheat varieties viz. Sourav and Prodip in in vitro condition. Seed health test of the collected wheat seed revealed that the seed was associated with six different fungi viz. Bipolaris sorokiniana, Fusarium sp., Aspergillus spp., Trichoderma viride, Penicillium sp. and Rhizopus sp. Wheat seeds were treated by dipping separately into different plant extracts of 1:1, 1:2 and 1:3 dilutions. Among the plant extracts, Lowsonia alba leaf extract was observed most effective followed by A. vasica, T. patula, V. rosea and A. paniculuta in reducing seed borne and seed associated mycoflora and also increased the germination rate and vigor index. J. bio-sci. 27: 23-30, 2019

scholarly journals The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 94
Author(s):  
Iman Mirmazloum ◽  
Attila Kiss ◽  
Éva Erdélyi ◽  
Márta Ladányi ◽  
Éva Zámboriné Németh ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Petri Dish ◽  
Seed Priming ◽  
Germination Percentage ◽  
Positive Effects ◽  
Carum Carvi ◽  
Early Seedling ◽  
Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

scholarly journals Peanut Seed Vigor Evaluation Using a Thermal Gradient

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Timothy L. Grey ◽  
John P. Beasley ◽  
Theodore M. Webster ◽  
Charles Y. Chen
Keyword(s):  
Seed Germination ◽  
Growth Curve ◽  
Thermal Gradient ◽  
Germination Rate ◽  
Petri Dish ◽  
Seed Vigor ◽  
Logistic Growth ◽  
Peanut Seed ◽  
High Maximum ◽  
Two Indices

Experiments conducted from 2007 to 2009 evaluated germination of 11 peanut runner-type cultivars. Germination was evaluated in Petridishes incubated over a thermal gradient ranging from 14 to 30°C at 1.0 C increments. Beginning 24 hr after seeding, peanut was counted as germinated when radicles were greater than 5 mm long, with removal each day. Germination was counted daily for seven days after seeding. Growing-degree day (GDD) accumulation for each temperature increment was calculated based on daily mean temperature for that Petri dish. Two indices were obtained from a logistic growth curve used to elucidate seed germination by cultivar: (1) maximum indices of germination and (2) GDD value at 80% germination (Germ80), an indication of seed vigor the lower the Germ80value, the greater the seed lot vigor. Based on the two indices, seed lots “AT 3081R”, “AP-3”, “GA-06G”, and “Carver” had the strongest seed vigor (Germ8026 to 47 GDD) and a high maximum incidence of germination rate (80 to 94%). Seed lots of “C99-R”, “Georgia-01R”, “Georgia-02C”, and “Georgia-03L” had inconsistent seed performance, failing to achieve 80% germination in at least two of three years.

scholarly journals Expression of MYB transcription factor gene ZmMYB59 affects seed germination in Nicotiana tabacum and Oryza sativa

2020 ◽  
Author(s):  
Kaihui Zhai ◽  
Guangwu Zhao ◽  
Hongye Jiang ◽  
Caixia Sun ◽  
Jingyu Ren
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Seed Germination ◽  
Transgenic Tobacco ◽  
Germination Rate ◽  
Myb Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Myb Transcription Factors ◽  
Vigor Index

Abstract Background: MYB transcription factors are involved in many biological processes, including metabolism, development and responses to biotic and abiotic stresses. In our previous work, a new MYB transcription factor gene, ZmMYB59 was induced by deep sowing and down-regulated during maize seed germination via Real-Time PCR. However, there are few reports on seed germination regulated by MYB proteins and the functions of ZmMYB59 remain unknown.Results: In this study, to examine its functions, Agrobacterium-mediated transformation was exploited to generate ZmMYB59 transgenic tobacco and rice. In T2 generation transgenic tobacco, germination rate, germination index, vigor index and hypocotyl length were significantly decreased by 25.0~50.9%, 34.5~54.4%, 57.5~88.3% and 21.9~31.3% compared to wild-type (WT) lines. In T2 generation transgenic rice, germination rate, germination index, vigor index and mesocotyl length were notably reduced by 39.1~53.8%, 51.4~71.4%, 52.5~74.0% and 28.3~41.5%, respectively. On this basis, relative physiological indicators were determined. The activities of catalase, peroxidase, superoxide dismutase, ascorbate peroxidase and proline content of transgenic lines were significantly lower than those of WT, suggesting that ZmMYB59 reduced their antioxidant capacity. As well, ZmMYB59 expression extremely inhibited the synthesis of gibberellin A1 (GA1) and cytokinin (CTK), and promoted the synthesis of abscisic acid (ABA) concurrently, which implied that seed germination was repressed by ZmMYB59 in hormone levels. Furthermore, cell length and cell number of hypocotyl/mesocotyl in transgenic tobacco and rice were notably decreased. Conclusions: Taken together, it proposed that ZmMYB59 plays a negative regulatory role during seed germination in tobacco and rice, which also contributes to illuminate the molecular mechanisms regulated by MYB transcription factors.

scholarly journals Peanut Seed Germination and Radicle Development Response to Direct Exposure of Flumioxazin Across Multiple Temperatures

2020 ◽  
Vol 47 (2) ◽  
pp. 89-93
Author(s):  
Nicholas L. Hurdle ◽  
Timothy L. Grey ◽  
C. Pilon ◽  
W. Scott Monfort ◽  
Eric P. Prostko
Keyword(s):  
Seed Germination ◽  
Regression Models ◽  
Field Application ◽  
Linear Regression Models ◽  
Peanut Seed ◽  
Application Rates ◽  
Arachis Hypogaea L ◽  
Direct Exposure ◽  
Table Data ◽  
Development Temperature

ABSTRACT Peanut injury in the field can occur from flumioxazin applied PRE, but this is associated with plants that have emerged, or are about to, emerge from soil. The direct effect of flumioxazin on peanut seed germination and radicle development has not been evaluated. Therefore, research was conducted to determine peanut seed radicle development response to flumioxazin at different concentrations (0.0, 0.01, 0.10, 1.0 and 10.0 ppb) when tested at multiple temperatures (20, 23, 26, and 29 C) in laboratory experiments on a thermogradient table. Data analysis indicated that flumioxazin concentration was not different from the nontreated control (0.0 ppb) for 0.01, 0.1, and 1.0 ppb for peanut germination. Flumioxazin at 10.0 ppb was different from all other treatments and the nontreated control. However, comparing linear regression models for each flumioxazin concentration across all temperatures indicated no differences for slope. These data indicate that when there is direct peanut seed exposure to flumioxazin at field application rates, there is no impact on germination and radicle development. Temperature was noted to affect radicle development greater than field application rates of flumioxazin. As temperature decreased, germination and radicle length was inhibited or decreased, respectively. Nomenclature: Flumioxazin, peanut, Arachis hypogaea (L.), radicle, seed germination

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