MATERIALS OF THE KONSTANTINOVSKAYA CULTURE FROM THE TEMERNITSKOYE FORTIFIED SETTLEMENT

В 2017-2018 гг. на территории Темерницкого городища в центральной части г. Ростова-на-Дону было обнаружено поселение энеолитического времени (константиновская культура). В статье отдельно рассмотрены эти материалы, представленные в подавляющем большинстве керамикой и кремневыми изделиями (рис. 2-4). Часть их находилась в переотложенном состоянии - в разрушенном слое или хозяйственных ямах первых веков н. э. Оставшаяся половина найдена в нижних практически стерильных слоях предматерика и в заполнении двух выявленных рвов энеолитического времени (рис. 1). Единственный сохранившийся участок культурного слоя исследован в западной части раскопа. Именно с этого участка происходят два шила, обогащенная медная руда и несколько фрагментов неорнаментированных стенок сосудов с примесью раковины. Находки из металла (2 шила, плоская капля металла, медная руда) проанализированы на микрофлуоресцентном рентгеновском спектрометре (рис. 5). По совокупности технологических, морфологических и орнаментальных признаков керамики энеолитический слой Темерницкого городища можно атрибутировать ранним этапом константиновской культуры Нижнего Подонья. Помимо эпонимного поселения наиболее близки к найденным материалы нижнего горизонта Ливенцовского поселения и слой 6 поселения Раздорское I. К исследованным материалам энеолитического времени Темерницкого городища хронологически близким является погребение, обнаруженное на территории грунтового некрополя Темерницкого городища. In 2017-2018 an Eneolithic settlement (attributed to the Konstantinovskaya culture) was discovered in the territory of the Temernitskoye fortified site in the center of Rostov-on-the-Don. The paper considers these materials represented mostly_by ceramics and flint items (Fig. 2-4). Some of them were redeposited and found in a disturbed layer or household pits dating to first centuries AD. The remainder items were found in bottom and practically sterile layers over the virgin soil and in the fill of two identified Eneolithic ditches (Fig. 1). The only surviving section of the cultural layer was examined in the western part of the excavation area. It yielded two awls, enriched copper ore and several undecorated walls of shell-tempered vessels. Metal finds (two awls, a flat metal drop, copper ore) were analyzed by an X-ray microfluorescence spectrometer (Fig. 5). Based on technological, morphological and decorative characteristics of the ceramics, the Eneolithic layer of the Temernitskoye fortified settlement can be attributed to the early stage of the Konstantinovskaya culture of the Lower Don region. Besides the eponymic settlement, the materials retrieved from the lower horizon at the Liventsovka settlement and layer 6 from Razdorskaya I are the closest to the finds. A burial found in the necropolis of the Temernitskoye fortified settlement is chronologically close to the examined Eneolithic materials found at the Temernitskoye settlement.

Taxonomic structure of the southern chernozem under application of biological preparations and different farming systems

The study of the taxonomic structure of the southern chernozem under influence of microbial preparations and various farming systems in the conditions of the southern steppe zone has been carried out. Metagenomic analysis of the microbiome of southern chernozem showed the presence of 174 genera representatives of prokaryotes, among which 17 are dominant, representing more than 1%. Farming systems cause changes in the taxonomic structure of the microbiome in comparison with virgin soil in the steppe zone. The influence of microbial preparations of complex action on the qualitative and quantitative composition of the microbiome of southern chernozem and increasing under no-till farming system has been established. The maximum content of water-soluble organic matter was found in virgin soil, which has higher amount of plant residues, up to 491 mg/kg of soil. Under the influence of farming systems its amount decreased in 4.2-8.5 times. The use of a complex of microbial preparations in the southern chernozem contributed to an increase in the amount of water-soluble fraction of humus depending on the farming system: 1.7 times with the conventional farming system and 3 times with no-till compared to the control without CMP.

ISOLATION, MOLECULAR CHARACTERIZATION OF DIAZOTROPIC BACTERIA FROM VIRGIN SOIL FROM ARAKU VALLEY AND ASSESSMENT OF OF AMMONIA ACCUMULATION AT SALINE CONDITIONS

Nitrogen-fixing bacteria are widely distributed in nature where they reduce atmospheric nitrogen in soil or in association with plant. They have been found in a wide variety of terrestrial and aquatic habitats in both temperate and tropical regions of the word. Nitrogen-fixing bacteria are found in symbiotic associations with plants free living in soil. The objective of the present research was to isolate free living Nitrogen fixing bacteria from virgin soil samples in araku valley and assessment of their ammonia accumulation at saline conditions. 10 soil samples were collected in different place from virgin areas. For isolation of the free living nitrogen fixing bacteria, Nitrogen free media like Jensens Medium and Azotobacter Agar were used. Serially diluted soil samples were spread on the agar media and incubated for 48 hours. Eleven morphologically different bacteria were separated on made pure colonies on nutrient agar media. All bacteria were under go biochemical characterization which reveals that all these bacteria related to Azospirillum, Azotobacter and Clostridium. High ammonia liberating isolate MGN-10 was molecular characterizes as Azotobacter chroococcum and this soil application increase the plant growth in terms of growth parameters.

Integrative Effects of Treated Wastewater and Synthetic Fertilizers on Productivity, Energy Characteristics, and Elements Uptake of Potential Energy Crops in an Arid Agro-Ecosystem

Using wastewater in agriculture is a desirable alternative source of irrigation and is gaining attraction worldwide. Therefore, this study was designed to assess the effect of treated municipal wastewater (TWW) and groundwater (GW), along with half and full doses of the recommended NPK dose on the plant growth, total biomass, gross energy, and macro- and trace element content and uptake of safflower (Carthamus tinctorius L.), canola (Brassica napus L.), and triticale (X Triticosecale Wittmack) grown in old and virgin soil as potential bioenergy crops. The results showed that crops planted in old or virgin soil irrigated with TWW had higher values of plant height, leaf area per plant, total chlorophyll content, total biomass, and gross and net energy contents compared to those irrigated with GW grown in virgin soil. Similarly, crops grown in old soil irrigated with TWW showed higher concentrations in dry matter and uptake for both macronutrients (N, P, and K) and trace elements (B, Zn, Mn, Cu, Cd, Pb, and Ni) compared to those planted in virgin soil and irrigated with GW. Furthermore, the application of the recommended half dose of NPK in old and virgin soil irrigated with TWW showed occasionally comparable results to that of a full recommended dose of NPK for most of the measured parameters. Importantly, the recommended half dose applied to old soil irrigated with TWW resulted in a significant improvement in all measured parameters compared to virgin soil irrigated with GW, along with a full recommended dose of NPK. Briefly, TWW can be used to irrigate crops grown for bioenergy purposes, since it did not pose any harmful effect for energy crops. In addition, it provides additional nutrients to soil and thus decreases the required rate of synthetic fertilizer by up to 50% without any significant decreases in the final production of crops.

Effect on CBR Values with Addition of Coir Geotextile and Marble Dust in Silty Sands

The subgrade is the foundation of pavement. The conventional method of replacing weak soil with good soil can cause an increase in the cost of a project. Due to this reason ground improvement techniques are much popular nowadays. The major goal of this research work is to compare California Bearing Ratio (CBR) values of the virgin soil and soil reinforced with coir geotextile in one layer and a combination of two layers at different heights from the top surface of the soil. To see the change in CBR values one layer of coir geotextile was reinforced at three different heights (i.e., H/3; H/2 and 2H/3). After that, the effect on CBR values by reinforcement of combination of two layers of coir geotextile at different heights (i.e., H/3 and H/2; H/2 and 2H/3; and H/3 and 2H/3) from the top surface of the soil was studied. Thereafter, the soil is replaced by various percentages of marble dust ranging from 10% to 25% with an increment of 5% and again CBR values of soil samples reinforced with one layer of coir geotextile and a combination of two layers of geotextile at three different heights were compared with virgin soil. The test results reviewed that the maximum dry density (MDD) decreased and optimum moisture content (OMC) increased with the replacement of marble dust in the soil. The CBR test results specify an enhancement of the value of CBR with the addition of coir geotextile and marble dust. The maximum value of CBR is obtained when one layer of coir geotextile was introduced at the height of H/3 and in the case of a combination of two layers of coir geotextile maximum CBR values is obtained when the coir geotextile was introduced at a height of H/3 and 2H/3 from the top surface of the soil.

Monitoring the content of manganese, zinc, and cobalt in Haplic Chernozem

The paper uses the materials of local agroecological monitoring of arable soils of the Belgorod region on the content of manganese, zinc, and cobalt in haplic chernozem of the steppe part of the Belgorod region. The content of manganese, zinc and cobalt in the arable horizon was 1.42, 1.22 and 1.23 times higher than in the parent rock, respectively. A strong correlation was established between the total contents of zinc and cobalt in the soil profile (r=0.77), and the bonds of manganese with zinc and cobalt had the strength of an average value (r=0.59 and r=0.55). The content of mobile forms of the studied trace elements in haplic chernozem is at a low level. The main reason for the shortage of mobile forms of manganese, zinc and cobalt is their low background content in virgin soil. Keywords: HAPLIC CHERNOZEM, GROSS CONTENT, CONTENT OF MOBILE FORMS, MANGANESE, ZINC, COBALT

Agrogenic changes in the properties of dark chestnut virgin and arable soil with different use and irrigation

In the article, it was provided a comparative analysis of the ecosystem significance of soil characteristics transformed as a result of changes in the use conditions. At the three monitoring stationary sites in the Chaplinka district of the Kherson region, we studied the properties of dark chestnut soil (Haplic Kastanozem) on virgin lands (a biosphere reserve "Askania-Nova") and with more than a hundred years of use in agricultural production without irrigation and with a 50-year irrigation with water from the Kakhovsky trunk canal. Changes in the salt regime of the soil and the composition of exchangeable cations were established. In irrigation the total content of water-soluble salts up to 0.14-0.21% (toxic salts ‒ up to 0.06-0.15 %) was increased. The composition of salts was changed, their migration and accumulation in the lower soil layers. Under the influence of fresh water irrigation the process of dealkalinization in the dark chestnut soil took place. The involvement of virgin soil in agricultural use led to a decrease in the total content of humus and the transformation of its group composition. The content of humic acids (HA) was decreased. The content of fulvic acids (FA) was increased. The HA/FA ratio in the 0-10 cm layer changed from 2.6 (in virgin soil) to 1.6 in irrigated soil. The plowing and agricultural use of the dark chestnut soil led to a change in the state of the microbial cenosis. The number of microorganisms assimilating mineral and organic nitrogen, actinomycetes, oligotrophs, and eutrophs were increased. With irrigation the degree of transformation of the microbial cenosis increases. The indicator of biological degradation of irrigated dark chestnut soil was 4 %. This corresponds to a low level of degradation. A set of 10 indicators of the quality of the studied soil was proposed for calculating the level of its provision of ecosystem services (supplying and supporting). The algorithm and calculation results are presented. The soil of all monitoring sites of Markeev station is characterized by a high level of provision of ecosystem services. In virgin soil it was 8.3 points; in arable soil without irrigation it was 7.2, and with irrigation it was 7.5 points.

Comparative study of microbial inoculants of cultivated and virgin soils of Nilgiri Biosphere for plant growth promotion

In virgin soils, microorganisms and plants live in harmony and both are dependent on each other for their livelihood. Absence of cultivation practices, undisturbed soil condition, high organic matter condition and other favourable conditions enables to flourish beneficial microbes. The research work was started to identify beneficial microbes from undisturbed virgin soils of Nilgiri biosphere with the ability to grow under low pH and under low temeperature conditions. Bio-inoculants viz., Azospirillum, Phosphobacteria, Azotobacter, Rhizobium and pseudomonas were obtained from cultivated and virgin soil samples of Nilgiris biosphere. When compared with type cultures, virgin soil isolates of respective inoculants have recorded better results in promoting plant dry weight in paper towel method. In cross streak assay, selected isolates found to be compatible with each other. In lignite carrier base formulation, the inoculants have reached a maximum population level of 107 and phosphobacteria reached 108 level. The population remained steady at this level up to 3 months. In the field trial studies conducted, the treatment of Azospirillum + Azotobacter + Phosphobacteria + Pseudomonas + 75% RDF has recorded maximum population of all the inoculants at 45th day after sowing. However, the maximum yield was observed in 100% RDF and bio-inoculant consortium applied treatment. This was closely followed by 75% RDF and bio-inoculant consortium applied plots. The results of the field trial have shown that bio-inoculant consortium along with 75% RDF application will lead to maximum yield with 25% saving in chemical fertilizer application.

Impacts of Long- and Short-Term of Irrigation with Treated Wastewater and Synthetic Fertilizers on the Growth, Biomass, Heavy Metal Content, and Energy Traits of Three Potential Bioenergy Crops in Arid Regions

The availability of suitable water is an important factor for increasing the cultivated areas and sustainability in arid (i.e., less than 200 mm precipitation per year) and semiarid regions (i.e., 200–700 mm precipitation per year). Therefore, this study aimed to analyze the impact of treated wastewater (TWW) and groundwater (GW) as well as synthetic fertilizers (50% and 100% of the recommended NPK dose; 150–150–60 kg N–P2O5–K2O ha−1) on the growth, biomass, energy traits, and macro and trace elements of maize (Zea mays L.), sorghum (Sorghum bicolor L.), and pearl millet (Pennisetum glaucum L) grown in old cultivated (first location; L1) and virgin soil (L2 and L3) as potential bioenergy crops. The soil in L1 has been irrigated with treated wastewater for the last 15 years and continued to be irrigated with treated wastewater in this investigation. The virgin soil was divided into two parts: the first part was irrigated with TWW, and the second part was irrigated with GW. The experiments were laid out in a split-plot with a randomized complete block design with water treatments (TWW in old and virgin soil, and GW in virgin soil) in main plots, and the two treatments of fertilization (50% and 100% of the recommended NPK dose) were distributed randomly in subplots. Compared with the crops irrigated with GW, the crops irrigated with TWW, whether grown on old or virgin soil, showed higher plant height, total chlorophyll content, leaf area per plant, total biomass, energy content, and gross energy with low ash. They also contained higher (but lower than permissible limits) concentrations of macro-elements (NPK) and trace elements (Fe, Mn, Cu, Zn, Cd, Pb, Ni, and Co). In addition, the application of a 50% recommended dose of NPK with TWW showed equivalent results to a 100% recommended dose of NPK on all measured parameters with few exceptions. In conclusion, the TWW can be used to irrigate field crops allocated for bioenergy production in arid regions because it does not harm the plants and environment. In addition, the 50% recommended dose of NPK fertilizer exerted no negative effects on the growth and energy production of field crops, thereby protecting the environment and reducing the leaching of excessive fertilizers into GW.

‘Fact’ as a Lexico-Syntactic Marker of the Character’s Linguistic Personality

The role of the attitude in the formation of the lexical-syntactic marker of the character’s linguistic personality is considered. An attempt is made to show how, due to the attitude, the same word (namely, the word ‘fact’) in different syntactic uses serves to create a lexical-syntactic marker used by word artists in order to identify and characterize different characters as linguistic personalities. The relevance of the study is to demonstrate that considering the pragmatic level of a linguistic personality makes it possible to differentiate different characters in whose speech the same lexical-syntactic marker prevails. The novelty of the research lies in the explanation of how the lexical-syntactic marking of the linguistic personality of different characters is carried out on the basis of the same verbal element. Based on the play by M. Gorky “Summer Residents” (Basov’s linguistic personality) and M. A. Sholokhov’s novel “Virgin Soil Upturned” (Davydov’s linguistic personality), it is shown that the speech individualization of different characters through the word ‘fact’ is due to the inclusion of this word in different dominant varieties of lexicon the syntactic marker is a ‘fact’, correlation with different attitudes both “from the author” and “from the character”, unequal participation in character speech cooperation.