Combined conservative treatment of a recurrent mandibular keratocyst by enucleation, diode laser, 5-Fluorouracil and dental implants: a case report with 18 years-follow up

In 2017, the World Health Organization classified the odontogenic keratocyst as a developmental odontogenic cyst. The posterior parts of the mandible, especially the angle and the ramus, are the most involved sites. Due to their high recurrence rate, keratocysts are managed surgically with careful complete excision. Additionally, chemical solutions such as Carnoy's solution, modified Carnoy's solution, and 5-Fluorouracil have been associated with surgical treatment. Diode lasers generate diverse wavelengths that have photothermal and photochemical special properties and could have some effects on the lining epithelium remnants of the cyst. In this paper, we discuss a case of mandibular recurrent odontogenic keratocyst treated with 5-Fluorouracil topical application after enucleation and diode laser application with 18 years follow-up and teeth replacement with dental implant. 5-Fluorouracil and diode laser have been found to be very effective in the treatment of keratocysts.

The Mitotic Index of Cajanus cajan from Kisar Island, in the Southwest of Maluku

The mitotic index of the roots of pigeon pea can be the basis for determining the growth of pigeon pea. The purpose of this research was to determine the time of root cell division, to observe the mitotic phases, and to determine the mitotic index of pigeon pea root cells. The preparation of the pigeon pea was carried out for 4 days to grow the roots. The roots were cut off at 08.00, 08.15, and 08.30 WIT (Eastern Indonesian Time). The roots were cut 0.5-1cm. Carnoy’s solution was used as the fixative solution using the Squash technique. The prepared roots were then observed using an Olympus cx-22 microscope and an OptiLab camera with a magnification of 100x40. The data were descriptively analyzed to describe the images of mitotic phases and the mitotic index presentation in the root cells of pigeon pea. The results of this research showed that the cell division of the pigeon pea roots began at 08.00 WIT, which was marked by the presence of a lot of prophase. The next phases that appeared were prometaphase, metaphase, and anaphase which occurred from 08.15 to 08.30 with different numbers. The highest mitotic index occurred at 08.15, when most of the root cells underwent metaphase. This study succeeded in revealing that the optimum time for pigeon pea root cell division is 08.15 WIT. In the future, this research can help pigeon pea farmers in Southwest of Maluku to carry out vegetative reproduction which is closely related to this mitotic study.

Genetic stability in interspecific hybridizations of wheat populations determined by meiotic index and pollen viability

The objective of this study was to evaluate genetic stability by determining the meiotic index (MI) and pollen viability in F1RC2 segregating populations, obtained by the crosses between wheat commercial cultivars and synthetic wheat, aimed at introgression/genetic resistance. For MI, three ears were collected per genotype, before anthesis, and they were fixed in Carnoy’s solution. The cytological slides were prepared by crushing the anthers and staining with 1% acetocarmine, and the normal tetrads and the ones with micronuclei were observed under a light microscope. For pollen viability, the ears were collected at the stage of mature pollen grain. The methodological procedure was similar to that of the tetrads, evaluating the pollen grains as viable, with little starch, with two pores, different sizes and non-viable. The pollen grain size was measured by the Axion Vision software. All the segregating populations with MI above 90% and high pollen viability (above 85%) reflected a stable meiotic behavior. About the variation in size, the CIGM90.909/BRS 179 cross showed pollen grains with 58.43 μm and CIGM93.298/BRS Guamirim, 47.15 μm. According to the results, wheat segregating populations were considered meiotically stable and suitable for use in wheat breeding programs and for incorporation of new important genes.

Additional treatment with Carnoy solution in surgical therapy of ameloblastomas: Case report

Introduction: Ameloblastoma is a benign neoplasm characterized by the proliferation of odontogenic epithelium that mainly affects the gnathic bones and, due to its invasive and expansive growth, presents high rates of recurrence to surgical treatment. Among the most conservative treatments are enucleation and marsupialization; among radicals, resections are more widespread. Objective: The objective is to present, through a case report, conservative surgical treatment with enucleation followed by the use of the Carnoy solution. Case report: A 24-year-old male patient arrives at the outpatient clinic of Hospital da Restauração with painful complaints of mild and constant intensity in the region of the left mandibular angle, with an evolution of three weeks. After a panoramic X-ray, the presence of the included 38 tooth was associated with an extensive unilocular radiolucent lesion, surrounding the angle and mandibular ramus. Preoperative examinations were performed for incisional biopsy. Histopathological diagnosis was unicystic ameloblastoma. In view of the histopathology obtained, we opted for enucleation of the lesion with concomitant use of direct Carnoy solution in the region of the lesion. Discussion: The choice of therapeutic behavior depends on the size, type of lesion, location and histopathology. After the surgical decision, a radiographic clinical follow-up is necessary to assess possible recurrences. Carnoy's solution is a cauterizing agent with moderate tissue penetration, rapid local fixation and hemostatic action, whose surgical use in cystic lesions has occurred since the beginning of the 20th century. Conclusion: Conservative treatment with the enucleation technique followed by complementary therapy using Carnoy's solution proved to be quite effective.

Histology of Mice (Mus Musculus) Liver Tissue Fixed with Carnoy's Solution With Variation of 4 Hours, 8 Hours and 12 Hours

Histopathological examination is a routine examination process for each tissue in the anatomic pathology laboratory. The stages in making histology preparations are fixation stages. The type of solution that can be used as a fixative solution other than 10% NBF is Carnoy's solution. Carnoy's solution is a fixative solution with a relatively fast fixation process of about 1-4 hours. The advantages of Carnoy's solution can lyse erythrocytes and dissolve lipids, have the ability to maintain cell nuclei, and retain glycogen. The purpose of this study was to determine the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using Carnoy's solution with time variations of 4 hours, 8 hours, and 12 hours. This is an observational research with descriptive research design criteria. The results of the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using the 10% NBF solution group obtained 100% good preparations. In the Carnoy solution group with a time of 4 hours, it gave 2.2% results of poor preparations and 97.78% of good preparations. While the treatment group with Carnoy's solution with a time of 8 hours obtained as many as 11.11% of preparations that were not good and 88.89% of good preparations. and in the Carnoy solution group with a time of 12 hours, there were 44.44% of the poor preparations and 55.56% of the good preparations.

Interference of fixatives and fixation period on the morphologic analysis of ovarian preantral follicles

Summary Ovine ovarian fragments (3 × 3 × 1 mm) were fixed in neutral buffered formalin (NBF), Carnoy’s solution (CAR), Davidson’s solution (DAV), or paraformaldehyde (PFA) for 12 h or 24 h. After this fixation time, each fragment was prepared for histological analysis. Although fixative and fixation period did not affect follicular and stromal cells density, the percentages of morphologically normal primordial and primary follicles was affected by the fixative type and period of fixation. Paraformaldehyde was not indicated as a fixative for ovarian fragments. Formalin was a suitable fixative only when the period of fixation was 12 h, while Carnoy was efficient after a fixation period of 24 h. In conclusion, the most indicated fixative for the morphological evaluation of ovarian preantral follicles was DAV, regardless of the fixation period, that is 12 or 24 h.

14-Year evolution odontogenic keratocyst: Case report

Odontogenic keratocyst is a developmental odontogenic cyst that is usually diagnosed in routine radiographs in the early stages. This fact increases the dentist’s responsibility for its diagnosis, and the professional should pay attention to all maxillomandibular complex and not only to the teeth. There is no standard protocol for the treatment of odontogenic keratocyst. However, surgical resection is recommended because of its high recurrence rate, especially in those cases with extensive bone destruction. The enucleation followed by Carnoy’s solution application has been shown to be a good alternative to resection and pointed out as the conservative method associated with the lowest recurrence rates in the treatment of odontogenic keratocyst. This study aimed to report a case of an extensive odontogenic keratocyst with more than 14 years of evolution that had not been diagnosed in three previous different panoramic radiographs and was treated with enucleation and application of Carnoy’s solution.

14-Year evolution odontogenic keratocyst: Case report

Odontogenic keratocyst is a developmental odontogenic cyst that is usually diagnosed in routine radiographs in the early stages. This fact increases the dentist’s responsibility for its diagnosis, and the professional should pay attention to all maxillomandibular complex and not only to the teeth. There is no standard protocol for the treatment of odontogenic keratocyst. However, surgical resection is recommended because of its high recurrence rate, especially in those cases with extensive bone destruction. The enucleation followed by Carnoy’s solution application has been shown to be a good alternative to resection and pointed out as the conservative method associated with the lowest recurrence rates in the treatment of odontogenic keratocyst. This study aimed to report a case of an extensive odontogenic keratocyst with more than 14 years of evolution that had not been diagnosed in three previous different panoramic radiographs and was treated with enucleation and application of Carnoy’s solution.