Cockayne Syndrome-Associated CSA and CSB Mutations Impair Ribosome Biogenesis, Ribosomal Protein Stability, and Global Protein Folding

Cockayne syndrome (CS) is a developmental disorder with symptoms that are typical for the aging body, including subcutaneous fat loss, alopecia, and cataracts. Here, we show that in the cells of CS patients, RNA polymerase I transcription and the processing of the pre-rRNA are disturbed, leading to an accumulation of the 18S-E intermediate. The mature 18S rRNA level is reduced, and isolated ribosomes lack specific ribosomal proteins of the small 40S subunit. Ribosomal proteins are susceptible to unfolding and the CS cell proteome is heat-sensitive, indicating misfolded proteins and an error-prone translation process in CS cells. Pharmaceutical chaperones restored impaired cellular proliferation. Therefore, we provide evidence for severe protein synthesis malfunction, which together with a loss of proteostasis constitutes the underlying pathophysiology in CS.

I Dress For Me: A Photographic Exploration of Clothing, Age and Identity

For this major research project, I Dress for Me, I investigated the relationship between clothing and life narratives for senior women through interviews and documentary-style photographs of the women’s wardrobes. Based on theories of identity and everyday dress by Irving Goffman and Efrat Tseëlon; fashion and age by Julia Twigg; and the practice of wardrobe interviews by Sophie Woodward, I set out to draw upon the life experiences of participants to gain a better understanding of how an individual’s relationship with clothing changes over time. The motivation for this project was to create a diverse representation of senior women within fashion. The result was gaining intimate accounts of women’s experiences and relationships with clothing and how they connected to life transitions, the aging body, life narratives and memory. Each woman’s relationship with clothing is complex and layered, as shown through their varied and diverse wardrobes that reflect their life narratives.

I Dress For Me: A Photographic Exploration of Clothing, Age and Identity

For this major research project, I Dress for Me, I investigated the relationship between clothing and life narratives for senior women through interviews and documentary-style photographs of the women’s wardrobes. Based on theories of identity and everyday dress by Irving Goffman and Efrat Tseëlon; fashion and age by Julia Twigg; and the practice of wardrobe interviews by Sophie Woodward, I set out to draw upon the life experiences of participants to gain a better understanding of how an individual’s relationship with clothing changes over time. The motivation for this project was to create a diverse representation of senior women within fashion. The result was gaining intimate accounts of women’s experiences and relationships with clothing and how they connected to life transitions, the aging body, life narratives and memory. Each woman’s relationship with clothing is complex and layered, as shown through their varied and diverse wardrobes that reflect their life narratives.

Like Two Fountains

Chapter 1 focuses on the aging body, and particularly on aspects of gender and sexuality of the aging body. Following a discussion of ancient medical theories regarding aging processes and their echoes in rabbinic texts, the chapter analyzes two rabbinic accounts regarding biblical figures whose bodies managed to defy the laws of aging. The first part of the chapter analyses the narrative of Abraham and Sarah’s return to their youth (BT Baba Metzi‘a 87a) and the vicissitudes through which their bodies went before and after the birth of Isaac, whereas the second part examines one story about King David’s sexuality in his old age (BT Sanhedrin 22a). The chapter argues that through the use of semi-mythical figures, the rabbis reveal both their desire to undo the processes of aging and their profound conviction that aging must ultimately be accepted.

COMPARATIVE CHARACTERISTICS OF THE EFFECT OF SOME INDUSTRIALLY SIGNIFICANT MEDICINAL HERBS ON THE CARDIOVASCULAR AND LYMPHATIC SYSTEMS OF AN AGING ORGANISM

The purpose of this study was to identify the corrective properties of a number of medicinal herbs for age-related changes in the aging body. In an experimental study on 8 groups of old animals, a number of medicinal herbs of the Kazakh flora were studied: Ziziphora Bunge, Almaty hawthorn, St. John's wort, Echinacea purpurea, kopeechnik, badan thick-leaved on the parameters of the cardiovascular and lymphatic systems. All the medicinal herbs tested by us affected the animal body to some extent and, according to the studied indicators, showed a positive effect in the direction of a younger organism. There was an increase in lymph flow, diuresis, weight loss, changes in the indicators of swellability, blood and lymph viscosity, blood pressure and heart rate. The data from the screening study allowed us to further combine them into a phytocomposition for subsequent studies in order to correct changes in the old body.

Advances in Understanding of the Role of Lipid Metabolism in Aging

During aging, body adiposity increases with changes in the metabolism of lipids and their metabolite levels. Considering lipid metabolism, excess adiposity with increased lipotoxicity leads to various age-related diseases, including cardiovascular disease, cancer, arthritis, type 2 diabetes, and Alzheimer’s disease. However, the multifaceted nature and complexities of lipid metabolism make it difficult to delineate its exact mechanism and role during aging. With advances in genetic engineering techniques, recent studies have demonstrated that changes in lipid metabolism are associated with aging and age-related diseases. Lipid accumulation and impaired fatty acid utilization in organs are associated with pathophysiological phenotypes of aging. Changes in adipokine levels contribute to aging by modulating changes in systemic metabolism and inflammation. Advances in lipidomic techniques have identified changes in lipid profiles that are associated with aging. Although it remains unclear how lipid metabolism is regulated during aging, or how lipid metabolites impact aging, evidence suggests a dynamic role for lipid metabolism and its metabolites as active participants of signaling pathways and regulators of gene expression. This review describes recent advances in our understanding of lipid metabolism in aging, including established findings and recent approaches.

Pendampingan Pastoral Kepada Lanjut Usia di HKBP Letare Ciledug

ABSTRACT Pastoral Care For Elderly at The Church of HKBP Letare Ciledug. This research is intended to give attention to elderly members of the church at the HKBP Letare Ciledug. HKBP Letare Ciledug is only 11 years old, but many of it’s members are elderly. Seen from an increasingly old age, physical and mental conditions that are declining, experiencing many crises and problems. The elderly crave ther ettention of those closest to them, especially from the church. The authors conducted research and want to provide servis through pastoral assistance that is sustaining and nurturing. The function of sustaining is to help elderly people who are sick or injured in order to survive overcoming the conditions experienced. Function of nurturing is to help the elderly develop the potential given by God to overcome the problems. Sampling was carried out for five elderly members of the church at the HKBP Letare Ciledug. The five members of the congregation interviewed represented the elderly congregation, having different struggles and problems, but had the same problem: limited mobility and activity due to illness and an aging body. This struggle has not been touched by Church service. With pastoral care services, through sustaining and nurturing, whic is done in the form of Group Counseling, by : Ministry of Word and Sacrament, Sermons, Prayer and Whorship, able to help elderly to mature and accept their life problems. In the future, church services to the elderly will develop and advance in the church of HKBP Letare Ciledug.

Comparison of DNA methylation clocks in black South African men

Aims: DNA methylation clocks are widely used to estimate biological age, although limited data are available on non-European ethnicities. This manuscript characterizes the behavior of five DNA methylation clocks in 120 older black South African men. Methods: The age estimation accuracy of the Horvath, Hannum and skin and blood clocks and the relative age-related mortality risk and predicted time to death portrayed by the PhenoAge and GrimAge biomarkers are investigated, respectively. Results: The results confirm the tendency of DNA methylation clocks to underestimate the biological age of older individuals. GrimAge more accurately characterizes biological decline in this African cohort compared with PhenoAge owing to the unique inclusion of smoking-related damage in the GrimAge estimate. Conclusions: Each clock provides a different fraction of information regarding the aging body. It is essential to continue studying under-represented population groups to ensure methylation-derived indicators are robust and useful in all populations.