APPLICATION OF OJIP CHLOROPHYLL FLUORESCENCE TRANSIENT ANALYSIS TO STUDY THE POSTHARVEST CHANGES IN PHOTOSINTHETIC APPARATUS IN CUT FOLIAGE SPECIES

Chlorophyll fluorescence (ChlF) is a non-invasive technique that can be potentially used in postharvest research to gain useful information on early responses to postharvest stresses. This study was conducted to validate the application of ChlF transient analysis in determining the postharvest changes in photosynthetic apparatus in three ornamental foliage species, i.e., Cordyline fruticosa ‘Willy’s Gold’ and ‘Rubra’, Dracaena sanderiana ‘White’, and Nephrolepis exaltata. Salicylic acid (100 and 300 mg·L−1), glucose (10 g·L−1), and their combinations were used as holding solutions with control treatment (distilled water) at room temperature (25±2°C). Vase life was evaluated using OJIP analysis. OJIP parameters, i.e., specific energy fluxes per reaction center (ABS/RC, TR/RC, ET/RC, and DI/RC), flux ratios (maximum quantum yield of primary photochemistry-φPo), electron transport efficiency (ψo), and quantum yield of electron transport (φEo), and performance index (PI) were recorded every other day, using a fluorometer (FluorPen 100). Leaf chlorophyll contents of all species and anthocyanin contents of two cordyline cultivars were determined. Data were subjected to ANOVA in a completely randomized design. Mean separation was done by DMRT (p ≤ 0.05). Clear variations in ChlF were observed in every foliage species with the time. OJIP analysis showed species-depended variations. The higher ABS/RC and DI/RC were recorded for D. sanderiana and N. exaltata compared to the PI of those species. At the end of the experiment, the chlorophyll contents were decreased, while anthocyanin contents were increased. Consequently, chlorophyll fluorescence changes in photosynthetic apparatus can be used for the prediction of the postharvest stresses and longevity of cut foliage.

SENSORY QUALITY OF QUAIL EGGS FED WITH CORIANDER FLOUR (CORIANDUM SATIVUM LINN) IN THE FEED

One of the sources of animal protein for the community can be obtained from quail eggs. Quail eggs have nutritional value that is not inferior to other poultry eggs. Feed is one of the factors that can affect egg quality. Coriander is a spice that contains essential oils that are beneficial for the body when consumed. So it is hoped that the presence of this essential oil can help improve livestock growth and produce quality quail eggs sensory. This study aimed to examine the effect of giving coriander flour to quail feed on the sensory quality of eggs. The study was conducted for 30 days. The livestock used were Japanese quail aged 30 days with 100 heads. The feed used is commercial feed and additional coriander flour. The design used was a completely randomized design with 4 treatments and 5 replications for each unit of 5 birds with treatment R0 = commercial feed +0% coriander flour, R1 = commercial feed + 0.5% coriander flour, R2 = commercial feed + 1% coriander flour, R3 = commercial feed + 1.5% coriander flour. Data were analyzed using Kruskall Wallis and Duncan's multiple range test. The results showed that the administration of coriander flour had a significant effect (P<0.05) on the hedonic test of egg white color and texture hedonic quality test, while for the egg yolk color, taste, aroma variables were not significantly different (P>0.05). This study concludes that organoleptic administration of 1% coriander flour can increase the panelists' preference for egg whites and improve the texture of quail eggs.

EFFECTS OF THE INCLUSION OF STAR GOOSEBERRY DRIED LEAF EXTRACT IN RATION ON EGG ORGANOLEPTIC VALUES OF LOCAL DUCK

Duck egg is known to have an unpleasant fishy odor which often becomes a factor reducing its acceptance in public. This odor is resulted from lipid oxidation which can be avoided by using an antioxidant. This study was aimed at assessing the effects of the inclusion of star gooseberry (Sauropus androgynus) dried leaf extract in ration on egg organoleptic quality of local duck. The study was conducted in a completely randomized design with 4 treatments and 4 replicates. Sensory test by 60 semi-trained panelists and hedonic quality and hedonic tests were conducted. Data were subjected to a Kruskal-Wallis test. Results showed that treatments gave significant effects (P <0.05) on aroma (hedonic), yolk color, and aroma (hedonic quality). It was concluded that the inclusion of star gooseberry dried leaf extract by 1,5% in ration improved the quality of egg yolk color, egg aroma, and consumers’ preference. A further study on the effects of DSGLE on meat quality and protein digestibility in ducks was recommended.

ISOLATION AND IDENTIFICATION OF LACTIC ACID BACTERIA (LAB) PRODUCING BACTERIOSIN-LIKE FROM SMOKED GIANT CATFISH (Arius thalassinus)

Bacteriocin was bacterial metabolite that have antimicrobial properties, so it had the potential to be used as food bio preservatives. Bacteriocin was produced by lactic acid bacteria (LAB), one of the sources of which was from smoked fish products. Some regions in Indonesia produce various types of smoked fish from various types of fish, which were thought to contain bacteriocin-producing lactic acid; one of them was giant cathfish (Arius thalassinus). This study aims to obtain LAB isolates that have strong antimicrobial activity and have the potential to produce bacteriocin-like from smoked giant catfish (Arius thalassinus). The research method used an experimental method that analyzed descriptively. Based on the results, there were 15 isolates LAB isolated from smoked giant catfish. Three selected isolates showed strong antimicrobial activity inhibiting E. coli and S. aureus bacteria, and the most effective inhibiting Salmonella sp. One selected LAB isolates identified Pediococcus acidilactici suspected to produce pediocin bacteriocin-like, while the other two isolates identified Lactobacillus plantarum sp 1 and Lactobacillus plantarum sp 2 which suspected to produce plantaricin bacteriocin. Bacteriocin from the three isolates of LAB had characteristics stable to temperatures up to 121oC, stable in pH range 2-6, and bacteriocin activity increased with the addition of SDS (Sodium Dodecyl Sulfate) and EDTA (Ethylenediaminetetraacetic acid) surfactants. The conclusion was that the bacteriocin produced was stable at high temperature, low pH, and resistence in the presence of surfactants, so it had the potential to be developed as biopreservatives material in preserving fish-based foods

SWEET POTATO CREAM SOUP AS AN ALTERNATIVE HEALTHY BREAKFAST MENU

Breakfast is an important thing for everyone. Breakfast requires a complete nutritional composition. Cream of sweet potato soup is an alternative to a proper healthy meal. Sweet potato (Ipomoea batatas L) contains carbohydrates, vitamins, minerals, fiber, oligosaccharides, and other phytochemicals that are good for health. Sweet potato production is very abundant, but its utilization is not maximal yet. The purpose of this research was to produce sweet potato cream soup. The study was done by experimental method and organoleptic test by using Quantitative Descriptive Analysis (QDA) method and evaluation of the final product by using a hedonic method. The steps in this study include prescription cream soup analysis, focus group discussion (FGD), trial and evaluation with QDA method, the final product is tested receiving power by using hedonic test. The results showed cream of sweet potato soup has nutritional adequacy as a breakfast menu, has the characteristics of savory, shiny, sweet potato and spice. Cream of sweet potato soup is favored bay panels. Thus cream of sweet potato soup is perfect for a healthy breakfast.

REVIEW OF THE KINEROS MODEL FOR PREDICTING THE EFFECT OF LAND USE ON THE SURFACE RUN-OFF

Topics protection of catchment area relating to land use to support sustainability and environmental capacity is an interesting topic to be discussed scientifically. The increasing frequency of floods, droughts, landslides, and water crises are recently showed that the management of watersheds (DAS) has not been optimal. The Kineros Model for predicting the effect of land use on the surface run-off need to be improve in application. This paper has shown that the protection of natural ecosystems has a better impact than infrastructure development, especially about preventing inundation due to high surface runoff. This journal will strengthen the need for watershed-scale erosion prediction analysis because of its ability to define strategic areas of policy intervention to enable policymakers and the community to carry out recovery programs/activities more effectively and focused. The accuracy of determining the location of the policy has an impact on cost, time, and energy efficiency. Spatial calculation/analysis models combined with social and economic approaches will create a balanced and integrated sustainable development. The reviewer suggests a realistic combination of approaches, which combines the spatial aspects and technocratic calculations aspects of economic instruments that become balanced in a multidisciplinary concept.

SAPPAN (Caesalpinia sappanL.) EXTRACT-BASED LABEL FOR DETECTION OF CHICKEN MEAT SPOILAGE

Brazilin in sappan is sensitive to pH, which make it possible as indicator of food quality. This research aimed to investigate performance of sappan extract as colorant for indicator label applied in chicken meat packaging. The samples were stored at room and refrigerator temperature. They were then tested for color change of label RGB (Red Green Blue), pH and TVB-N (Total Volatile Base-Nitrogen) of the meat. Data were analyzed using ANOVA at significant level of 5%. Indicator label showed the reduced RGB during storage, while pH and TVB-N increased. In case of room temperature, the color change into pink occurred in day 3 with pH 6.72 and TVB-N 1.84 mg N/100g. Meanwhile, for samples at refrigerator temperature, the label color turned into pink in day 6, with pH 6.56 and TVB-N 2.09 mg N/100g. In this regard, indicator label could detect the depletion of chicken meat quality as indicated by color shift from yellow to pink when pH and TVB-N were at critical value. This result provides significant output for development of sappan extract for indicator of meat spoilage.

THE EFFECT OF CONCENTRATION AND FREQUENCY OF APPLICATION OF BANAN WEEVIL SOLUTION KEPOK ON RED CHILI

The purpose of the study is to determine the effect of concentration and frequency of application of banana weevil solution kepok against red chili plants is roofed with translucent ultraviolet plastic 70% which acts as a natural growth regulator. Research on the provision of banana weevil solution covered in 70% translucent ultraviolet plastic in open land has not been found before. The growing regulators from the gibberellin and cytokinin groups were able to boost the growth and yield of chili peppers. The method of using a random design of factorial groups, as the first factor is age consists of 9 levels, namely T1: age (14) HSPT, T2: age (14.21) HSPT, T3: age (14,21,28) HSPT, T4: age (14,21,28, 35) HSPT, T5 : age (14,21,28,35, 42) HSPT, T6: age (14,21,28,35,42,49) HSPT, T7: age (14,21,28,35,42,49,56) HSPT, T8: age (14,21,28,35,42,49,56,63) HSPT, and control (T0). The second factor is concentration consists of 3 levels, namely K1 (30%), K2 (45%), and K0 (0%). The results of the study obtained concentration of K1 (30%) with the amount of T6 administration is the best result in vegetative growth of the plant height change, the number of branches, and the width of the header, while the concentration of K1 (30%) give the best results on the length of the fruit, the dry weight of the fruit, and the diameter of the fruit from other treatments. Conclusion of the application of concentration and frequency of the sea of banana weevil kepok containing regulatory substances growing on red chili plants in the field covered with ultraviolet plastic roof significantly increases the growth and yield of red chili peppers.

THE POTENTIAL INHIBITION OF XANTHINE OXIDASE BY PHENOLIC AND FLAVONOIDS DATE (Phoenix dactylifera) WITH MOLECULAR DOCKING METHOD

Dates is fruit of palm trees that mostly grow in the Middle East. Dates contain phenolic acids and flavonoids that have antioxidants and potentially inhibit the ability of xanthine oxidase. The purpose of this research to determine the molecular interactions of xanthine oxidase by ligand of phenolic acids and flavonoids to inhibiting the production of uric acid. This research was conducted by site directed docking method. The size of the center of retardation used in this research is x = 26.569, y = 9.985, and z = 113.088 and the retardation volume of x = 14, y = 14, and z = 16. Inhibition by flavonoid and phenolic acid compounds has produces good inhibition strength shown by Gibbs free energy which is negative. The compound with the highest Gibbs free energy value is the anthocyanins compound which is -7.3 kCal / mol, the value is higher than the comparator ligand, allopurinol. Based on the bond analysis that is formed, the best compound in inhibiting xanthine oxidase is syringic acid.

CARCASS AND MEAT QUALITY CHARACTERISTICS OF WILD BOAR CROSSBREDS

Due to the growing interest in the production and consumption of wild boar meat, the quality attributes of meat products should be well established. To characterize the quality of wild boar cross meat, two males and two females reared in the same environment were slaughtered at an average of one year of age, and meat samples were was collected and analyzed for different parameters such as for moisture, pH value, protein, fat, iron, phosphorous, calcium, water holding capacity through the measure of cooking loss. Data obtained for live body weight, carcass biochemical characteristics, and meat quality traits were submitted to one-way analysis of variance (ANOVA) using a general linear model (uni-variate) in Statistical Package for Social Sciences (SPSS) computer program and the level of significance was determined observing the probability value (p) obtained through ANOVA of respective traits. To study the association among nutritive values and body parts, Pearson’s correlation coefficients were determined using in Statistical Package for Social Sciences (SPSS) computer program. The pH values were found to be in the range of 5.78 to 5.90. Moisture, total ash, fat, and protein contents were observed to be in the range of 71.38 to 73.57 %, 0.89 to 1.10 %, 4.34 to 5.54 %, and 16.73 to 18.84 % respectively. Iron, phosphorous, and calcium content were found to be in the range of 1.94 to 2.94 mg/100g, 20.17 to 37.57 mg/100g, and 170.68 to 190.88 mg/100g respectively whereas cooking loss was in the range of 31.59 to 26.88 %. Sensory evaluation of meat samples was examined by a group of panelists according to the hedonic rating test. The consumer preference was similar to both types of meat samples (male and female). The overall acceptance; however, were higher in female samples than in male samples due to boar taint evident during the cooking or eating of pork derived from non-castrated male pigs once they reach puberty. The comparison of wild crosses among available commercial pigs and indigenous pigs is warranted for further studies with an appropriate and defined production system to obtain lean meat as per the consumer preferences which could be extensively used in commercial pig production to provide high-quality pork.