scholarly journals Biodegradation of Methyl Parathion Using Pseudomonas stutzeri (MTCC 2643)

2020 ◽  
Vol 2 (2) ◽  
pp. 19-33
Author(s):  
Archana S ◽  
Surendran A ◽  
Thatheyus AJ

Pesticides applied in agricultural fields for crop protection result in the contamination of the environment. They also affect the flora and fauna as well as the quality of air, water and soil. Hence their remediation is of concern. Among different strategies available, microbial remediation is cost effective and ecofriendly. The present work is designed to test the efficiency of Pseudomonas stutzeri, a bacterial strain obtained from MTCC, IMTECH, Chandigarh, India. After treating with 50, 100, 150 and 200 ppm of methyl parathion, parameters like, orthophosphate released, pH and turbidity changes were monitored upto thirty hours. Degradation of 200 ppm concentration was confirmed by UV-Visible spectrophotometry and HPLC analysis. This strain effectively degraded methyl parathion and it can be used in the remediation of contaminated agricultural fields.

2020 ◽  
Vol 2 (4) ◽  
pp. 8-19
Author(s):  
Vaishali S ◽  
Surendran A ◽  
Thatheyus A.J

Pesticides are applied in agricultural fields for controlling pest population to achieve crop protection. But they cause damage to nontarget organisms and affect the quality of environment including water, air and soil. The present study has been designed to test the efficiency of Pseudomonas stutzerion the degradation of malathion. The bacterial strain was subjected to 50, 100, 150 and 200 ppm of malathion in minimal broth for 30 hours and changes in orthophosphate levels, pH and turbidity were monitored for every six hours. Efficiency of free and immobilized cells were compared for orthophosphate release. Influence of different sugars on degradation was also compared. Degradation of 150 ppm of malathion was confirmed with UV-Visible spectrophotometric analysis and HPLC analysis. The data were subjected to two way analysis of variance and the results are discussed.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Rahul Kumar ◽  
Leonardo Binetti ◽  
T. Hien Nguyen ◽  
Lourdes S. M. Alwis ◽  
Arti Agrawal ◽  
...  

AbstractKnowledge of the distribution of the aspect ratios (ARs) in a chemically-synthesized colloidal solution of Gold Nano Rods (GNRs) is an important measure in determining the quality of synthesis, and consequently the performance of the GNRs generated for various applications. In this work, an algorithm has been developed based on the Bellman Principle of Optimality to readily determine the AR distribution of synthesized GNRs in colloidal solutions. This is achieved by theoretically fitting the longitudinal plasmon resonance of GNRs obtained by UV-visible spectroscopy. The AR distribution obtained from the use of the algorithm developed have shown good agreement with those theoretically generated one as well as with the previously reported results. After bench-marking, the algorithm has been applied to determine the mean and standard deviation of the AR distribution of two GNRs solutions synthesized and examined in this work. The comparison with experimentally derived results from the use of expensive Transmission Electron Microscopic images and Dynamic Light Scattering technique shows that the algorithm developed offers a fast and thus potentially cost-effective solution to determine the quality of the synthesized GNRs specifically needed for many potential applications for the advanced sensor systems.


2019 ◽  
Vol 32 (1) ◽  
pp. 27-33
Author(s):  
Anshita Gupta

In India, Chhattisgarh is famous as Herbal state with its beautiful natural resources of forests and agricultural fields and value addition has been done so far in Chhattisgarh to maintain or improve the quality of the plants before the material reaches the industry. Presently, the herbal industry in Chhattisgarh is not very organized. The plant derived gums, mucilages from natural sources like carrageen an, thaumatin, lard, storax, agar, gum acacia, tragacanth and many more to name comply with many requirements of pharmaceutical excipients. These can be preferred for formulation development as being stable and involving less regulatory issues as compared to their synthetic counter parts. They can also be easily modified to meet the specific needs, thereby being a potent and economic vehicle for delivering active pharmaceutical ingredient in formulation. Thus our concerned and aims of the study is to throw light on the potential of natural excipients which can be proposed to be used as diluent, binder, disintegrant as well as lubricant in various types of formulations as they are biocompatible and capable of giving additional nutrition to the developed dosage form from indigeneous sources of Chhattisgarh state which could serve for cost effective and economical medicament and will enhance the value addition to the proposed herb and herbal species.


Author(s):  
Pushkar Yadav

Pesticides are majorly used in agricultural fields for crop protection but even after having agricultural benefits it also has negative acute toxic effects on human beings and environment. Accumulation of these toxic substances in the living life cycle prove to be a source of diseases and causes of deaths if accumulated in higher concentration. This research involves a method to detect the quantity of pesticides accumulate in our edibles which we consume, so as to ensure the quality of food we eat. For this purpose, a colorimetry method can be used which is the application of beer-Lambert law (1) that measures the concentration of solute by measuring the loss of intensity of light which is passing through the solution.


Author(s):  
I.N. Voronchikhina ◽  
◽  
A.G. Marenkova ◽  
V. S. Rubets ◽  
V. V. Pylnev

The results of elements development of varietal agrotechnics of a new high-potential line 238h of winter triticale presented. It was identified that under the conditions of 2020 the most cost effective fertilizer system is an early spring application of NPK (S) (15-15-15 (10)) at a dose of 200kg/ha. The profitability level of this fertilizer was 88,9%.


1998 ◽  
Vol 37 (4II) ◽  
pp. 873-882 ◽  
Author(s):  
Sarfaraz Khan Quershi

Growth in telecom infrastructure and provision of modern telecom services to consumers at a cost based tariff helps growth of national economy. Modern telecoms serve as the engine of growth of national economy. Following the global trends of liberalisation and deregulation in telecoms monopolies which have thus far been providing inefficient communication at a greedily high tariffs are falling apart. Mergers in telecoms are not for increasing the size of the monopoly but to provide more efficient and cost effective services to the consumers. In Pakistan the erstwhile T&T department played a needful role at its time. Conversion of the department into a corporation and then into a company were steps necessary for following the global trends. Need now is to continue this trend further, eliminate the monopolistic approach by allowing more players in the field thus permitting the market forces to decide the provision of better quality of modern services at competitive price.


Author(s):  
V. Z. Venevtsev ◽  
М. N. Zakharova ◽  
L. V. Rozhkova

Ryazan region annually receives stable yields of sugar beet roots 40 t/ha. Further growth of yields depends on balanced nutrition of plants cultivated hybrids, from the quality of the soil and of the phytosanitary State of sowing culture. Weed vegetation in wider spaced row crops of sugar beet in the initial periods of vegetation is high competition culture. The article presents the results of three studies on the effectiveness of the herbicide betanalnoj group, used to reduce contamination of sowing culture annual dicotyledonous weeds and increase the harvest of sugar beet roots. Studies conducted on experimental fields ISSA-branch FGBNU FNAC WIM (former AGRICULTURAL RESEARCH INSTITUTE in Ryazan). Soil: dark grey forest tjazhelosuglinistaja, humus content 4.0%, potassium and phosphorus-high pH is 5.8. Area of cultivated plots 50 m2, repetition, four sugar beet variety-Ocean. The predecessor-winter wheat. Under the autumn ploughing had made NPK120 under presowing cultivation-N60, SEV conducted seeder sowing machine. For crop protection herbicides were tested annually of sugar beet Betanal progress, UF-1.0 l/HA, Forte di Belvedere-1.0 l/HA, Betanal Max Pro-1.5 l/HA, Bajrang engineering works Super-1.5 l/HA, once applied to weeds. The harvest of sugar beet roots, take into account the square 10 m2 in 4-times repeated with each experimental plot by weighing machinery, processed data by ANOVA. The research found that studied herbicides efficiently at 87-92%, reduced infestation annual dicotyledonous weeds and increase the harvest of sugar beet roots to 29.9-44.1%


Author(s):  
Gwee Hoon Yen ◽  
Ng Kiong Kay

Abstract Today, failure analysis involving flip chip [1] with copper pillar bump packaging technologies would be the major challenges faced by analysts. Most often, handling on the chips after destructive chemical decapsulation is extremely critical as there are several failure analysis steps to be continued such as chip level fault localization, chip micro probing for fault isolation, parallel lapping [2, 3, 4] and passive voltage contrast. Therefore, quality of sample preparation is critical. This paper discussed and demonstrated a quick, reliable and cost effective methodology to decapsulate the thin small leadless (TSLP) flip chip package with copper pillar (CuP) bump interconnect technology.


2017 ◽  
Vol 6 (04) ◽  
pp. 5347 ◽  
Author(s):  
Omar B. Ahmed* ◽  
Anas S. Dablool

Several methods of Deoxyribonucleic acid (DNA) extraction have been applied to extract bacterial DNA. The amount and the quality of the DNA obtained for each one of those methods are variable. The study aimed to evaluate bacterial DNA extraction using conventional boiling method followed by alcohol precipitation. DNA extraction from Gram negative bacilli was extracted and precipitated using boiling method with further precipitation by ethanol. The extraction procedure performed using the boiling method resulted in high DNA yields for both E. coli and K. pneumoniae bacteria in (199.7 and 285.7μg/ml, respectively) which was close to control method (229.3 and 440.3μg/ml). It was concluded that after alcohol precipitation boiling procedure was easy, cost-effective, and applicable for high-yield quality of DNA in Gram-negative bacteria.


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