Occurrence of canine mammary and skin/ subcutaneous neoplasms in and around Thrissur district of Kerala during 2017-2020: A review of 265 cases

The study was conducted in 265 clinical cases of mammary and skin/ subcutaneous neoplasms in dogs presented to University Veterinary Hospitals Mannuthy and Kokkalai during a period of 36 months from October 2017 to September 2020. Mammary neoplasms were found more in females (51.7 per cent) than in males whereas skin and subcutaneous neoplasms were found more in male dogs (48.3 per cent). The maximum occurrence of neoplasms was recorded in the age group of eight to twelve years (38.5 per cent) whereas least occurrence was noticed in the age group up to four years (9 per cent). Labrador and Rottweiler breeds were found more affected with neoplasms (38 per cent each) with highest occurrence of mammary neoplasms in inguinal mammary glands (35.03 per cent) and highest occurrence of skin/ subcutaneous neoplasms on sites involving trunk region (14.06 per cent cases). Eighty-three per cent of the neoplasm cases in the present study were pet dogs with a greater number of dogs maintained in outdoor kennels and 17 per cent of neoplasm was found in free-roaming dogs rescued from streets. Out of total 265 dogs, 37.73 per cent dogs were found to be having commercial dog food as their main feed and 32 per cent dogs were fed with a mixed diet of homemade food and commercial dog food. Among the cases, 14.71 per cent dogs had a previous history of cancer surgery

Influence of Probiotic Supplementation on Health Status of the Dogs: A Review

Most commonly, pet dogs suffer from gastrointestinal (GI) diseases due to careless eating behaviors, such as eating food other than dog food; excess or insufficient nutrient intake of food leading to malnutrition, which could be harmful to dogs; a lack of digestive enzymes; food intolerance or allergies; infections; and/or breed-related hypersensitivities. Probiotics are live microorganisms that deliver health benefits to the host when administrated in an adequate amount. The possible mechanism behind probiotics’ beneficial effects could be their positive regulation of the host’s intestinal microbiota. Probiotics are reported to have therapeutic properties against canine GI and other diseases. The most suitable dosages and applications of probiotics have not been evaluated extensively. The present review summarizes current knowledge regarding the benefits of probiotics and the changes in canine microbiota during probiotic interventions. This literature review provides clinical evidence for probiotics’ beneficial effects in preventing or treating canine ill-health conditions. Based on current knowledge, subsequent researchers could develop or improve probiotics-based canine pharmacological products.

Ability of dog owners to identify their dogs by smell

Several studies report that olfactory cues play an important role in human life; humans are essentially able to recognize other family members and friends by their odors. Moreover, recent studies report that humans are also able to identify odors of non-conspecifics. The aim of this study was to determine whether dog owners are able to identify their dogs by smell and distinguish the odor of their own dogs from those of other dogs. A total of 53 dog owners (40 females and 13 males of different ages) volunteered to take part in this study. A number of the participants (17) owned 2 dogs; these owners took part in the study twice (i.e., working with only one dog at a time). Sterile gauze pads were used to collect odor samples from the dogs. Each pad was placed in its own sterile glass jar (750 ml) with a twist off lid until the experiment commenced. Participants were asked to identify their own dog´s odor from a line-up of 6 glass containers. This experiment demonstrated that dog owners are capable of identifying their dogs by smell on a significant level. Results of this study additionally suggested that male owners outperformed their female counterparts in the identification process. Moreover, dog owners whose dogs were housed outside had a higher success rate in identification than did participants who kept their dogs indoors with them. The dog owners found it easier to identify dogs that had been neutered, fed dry dog food and bathed less frequently. In general, younger dog owners tended to have more success when attempting to identify their dogs than did their older counterparts.

Survey evaluation of dog owners’ feeding habits in a household setting and comparison of FDA hygiene protocols on dog bowl bacterial contamination as evaluated by total aerobic cell counts.

In-home pet food handling and food dish hygiene practices can have adverse health impacts for both humans and pets. Safe food and dish handling guidelines are not easily evidenced for pet owners. The study was designed to investigate dog owners’ feeding habits and evaluate the impact of the Food and Drug Association (FDA) hygiene protocols on dog food dish contamination. Procedures and surveys were approved by NCSU-IACUC and -IRB. Pet feeding and food dish hygiene data were collected from 417 dog owner surveys and 68 food dish swabs. Total aerobic cell counts (TAC) were performed on 68 dishes and randomly assigned into Group A (FDA pet food handling and dish hygiene guidelines), Group B (FDA pet and human food handling and dish hygiene guidelines), or Group C (no guidelines). Hygiene protocols were instituted in-home for 1 week, followed by a second TAC and follow-up survey. Survey from dog owners-households indicated: 4.7% were aware of FDA pet food handling and dish hygiene guidelines; 36% have individuals < 13 years old and/or immunocompromised; 43% store dog food 0-5 feet from human food; 34% wash their hands after feeding; and 33% prepare their dog food on human food preparation surfaces. The hygiene protocols followed by Groups A and B resulted in significant decreases in food dish TAC (P<0.001; 1.40; p =0.026; 0.604, respectively), as compared to Group C ( p =0.373). Hot water (>160F) washing decreased TAC ( P p =0.005) over cold/lukewarm water. In the follow-up survey, 8% of Group A and B respondents reported likely to adhere to protocols long-term. This study suggests a need for pet food handling and dish hygiene guideline education to minimize bacterial contamination of dishes, especially in high-risk households.

PSV-B-22 Evaluation of whole soybeans as an ingredient in dog diets: Canine palatability of extruded diets with graded levels of dehulled whole soybeans

Increasing the amount of liquid fat in the ration during extrusion can negatively affect product density and product expansion. High-fat ingredients, such as whole soybeans (WSB), may increase energy density while avoiding production issues. In a preliminary extrusion project, the addition of graded levels of WSB on food processing was evaluated. The objective of this study was to determine the effect of incremental addition of whole soybeans in dry dog food on palatability. Experimental diets were extruded with 0%, 10%, 20%, and 30% WSB (WSB0, WSB10, WSB20, and WSB30, respectively). Palatability was determined with a 2-bowl test by beagle dogs (n = 20) for 2 days with each WSB diet compared to the WSB0. First choice preference and total food consumption were recorded for each dog. Individual intake ratios (IR) were calculated (intake of each diet/total intake). First choice (FC) was analyzed by a Chi2 probability, and the consumption of each diet was compared by a Wilcoxon signed rank test and a 2-way analysis of variance (2-way ANOVA). Dogs had greater (P &lt; 0.05) first choice for WSB diets relative to the WSB0, but there was no significant difference among treatments for the food consumption and intake ratio. When comparing WSB0 to WSB10, FC occurred in 13/40 occasions (P &lt; 0.05). The FC for WSB0 occurred in 12/40 occasions compared to WSB20 and in 11/40 occasions compared to WSB30 (P &lt; 0.05). Overall, dogs favored WSB diets over the control diet showing a higher score in the indication of aroma, but this did not result in higher consumption. In conclusion, palatability was not affected by the inclusion of WSB in the dry dog food.

Aflatoxicosis in dogs

Aflatoxins are toxic, naturally occurring bisfuranocoumarin compounds produced by certain strains of the moulds Aspergillus flavus, A. parasiticus and A. nomius. Aflatoxin metabolites cause hepatotoxicity by reacting with macromolecules (including DNA and proteins) to cause fatty liver or liver necrosis. Most cases involve dog food or, less commonly, ingestion of mouldy bread. Periodic outbreaks are reported in dogs, most recently at the end of 2020 to early 2021 in the US. Multiple dogs may be involved in incidents and the dogs usually present with gastrointestinal signs, lethargy, melaena and jaundice. Diagnosis is based on a history of possible ingestion and laboratory confirmation of aflatoxin(s) in suspect material. In the liver the typical histological changes are centrilobular necrosis of the liver and bile duct proliferation. Treatment of aflatoxicosis in dogs in supportive, with management of liver failure. Prognosis depends on the severity of liver damage, but mortality rates in dogs with aflatoxicosis are high.

Addition of dietary methionine but not dietary taurine or methyl donors/receivers to a grain-free diet increases postprandial homocysteine concentrations in adult dogs

Grain based ingredients are replaced in part by pulse ingredients in grain-free pet foods. Pulse ingredients are lower in methionine and cysteine, amino acid (AA) precursors to taurine synthesis in dogs. While recent work has investigated plasma and whole blood taurine concentrations when feeding grain-free diets, supplementation of a grain-free diet with various nutrients involved in the biosynthesis of taurine has not been evaluated. This study aimed to investigate the effects of supplementing a complete grain-free dry dog food with either methionine (MET), taurine (TAU), or methyl donors (choline) and methyl receivers (creatine and carnitine; CCC) on postprandial AA concentrations. Eight healthy Beagle dogs were fed 1 of 3 treatments or the control grain-free diet (CON) for 7 d in a 4 × 4 Latin square design. On d7, cephalic catheters were placed and one fasted sample (0 min) and a series of 9 post-meal blood samples were collected at 15, 30, 60, 90, 120, 180, 240, 300 and 360 min. Data were analyzed as repeated measures using the PROC GLIMMIX function in SAS (Version 9.4). Dogs fed MET had greater plasma and whole blood methionine concentrations from 30 - 360 min after a meal (P &lt; 0.0001) and greater plasma homocysteine concentrations from 60 - 360 min after a meal (P &lt; 0.0001) compared to dogs fed CON, TAU and CCC. Dogs fed TAU had greater plasma taurine concentrations over time compared to dogs fed CON (P = 0.02), but were not different than dogs fed MET and CCC (P &gt; 0.05). In addition, most AA remained significantly elevated at 6 h post-meal compared to fasted samples across all treatments. Supplementation of creatine, carnitine and choline in grain-free diets may play a role in sparing the methionine requirement without increasing homocysteine concentrations. Supplementing these nutrients could also aid in the treatment of disease that causes metabolic or oxidative stress, including cardiac disease in dogs, but future research is required.

Standardisation of alien invasive Australian redclaw crayfish Cherax quadricarinatus sampling gear in Africa

Freshwater crayfish are damaging invaders across southern Africa; however, monitoring techniques and efforts are disparate across the region as different sampling methods have been used. To develop a standard method for assessing redclaw crayfish Cherax quadricarinatus abundance, a survey was conducted to assess for differences in detection and catch per unit effort (CPUE) in Lake Kariba. Two sampling approaches were compared: opera traps baited with cooked maize meal historically used in crayfish surveys in Zimbabwe, and Promar collapsible traps baited with dry dog food, which have been used for assessments in South Africa and Swaziland. Baits were compared in the Barotse Floodplain in Zambia using the Promar trap. Detection probability (Pcapture) and CPUE were significantly lower for opera traps baited with cooked maize meal (Pcapture = 0.41; CPUE = 1.19 ± 0.24 ind.·trap-1·night-1) compared to the Promar traps baited with dry dog food (Pcapture = 0.67; CPUE = 4.53 ± 0.82 ind·trap-1·night-1). The Pcapture and CPUE for Promar traps baited with dog food (Pcapture = 0.89; CPUE = 4.29 ± 0.83 ind·trap-1·night-1) was significantly higher than for maize meal baited traps (Pcapture = 0.29; CPUE = 0.25 ± 0.17 ind·trap-1·night-1). Sex ratio and carapace length of crayfish sampled did not differ between sampling methods. Due to higher CPUE, the authors consider the Promar collapsible trap baited with dog food approach as the better method for determining crayfish population abundance and suggest that comparisons of abundance take this into consideration by applying conversion factors if different methods are applied.

Effect of Intraspecific Larval Aggregation and Diet Type on Life-History Traits of Dermestes maculatus and Dermestes caninus (Coleoptera: Dermestidae): Species of Forensic Importance

Introduction: Numerous studies have examined the effect of abiotic factors on the development and survival of Dermestes and their importance for forensic entomology. Dermestes maculatus is one of the most known beetle species associated with corpses and D. caninus has little biological information available and no case report records. To better understand the life-history traits of those species we evaluated the impact of diet type and intraspecific larval density. Methods: Adult beetles were collected from human remains and colonies were kept under controlled conditions (27.0 ± 1.0 °C, 55.0% RU, and 12:12 L:D) and F1 generation was used to collect eggs. Newly emerged larvae were separated according to the treatments, being the combination of larval density (1, 15, 30 individuals), food (dried dog food or dried pork) and contact (with or without). We used factorial-ANOVA to test the individual and combined effect of both larval densities and diet on dependent variables, followed by post-hoc Tukey test. Pearson correlations were carried out to evaluate the relationship between larval parameters for each species in each treatment. Results: Pork-based diet positively affected species fitness, with larvae being ca. 1.1 (D. caninus) and 1.7 (D. maculatus) times bigger and heavier than in dog food. Diet type also impacted the development time for both species. Conclusions: Data generated through the current study serve as a foundation for potential application of this species as an indicator of time of colonization in relations to a minPMI. However, validation is still needed to determine the accuracy and precision of these calculations.