Multiple New Paralytic Shellfish Toxin Vectors in Offshore North Sea Benthos, a Deep Secret Exposed

In early 2018, a large easterly storm hit the East Anglian coast of the UK, colloquially known as the ‘Beast from the East’, which also resulted in mass strandings of benthic organisms. There were subsequent instances of dogs consuming such organisms, leading to illness and, in some cases, fatalities. Epidemiological investigations identified paralytic shellfish toxins (PSTs) as the cause, with toxins present in a range of species and concentrations exceeding 14,000 µg STX eq./kg in the sunstar Crossaster papposus. This study sought to better elucidate the geographic spread of any toxicity and identify any key organisms of concern. During the summers of 2018 and 2019, various species of benthic invertebrates were collected from demersal trawl surveys conducted across a variety of locations in the North Sea. An analysis of the benthic epifauna using two independent PST testing methods identified a ‘hot spot’ of toxic organisms in the Southern Bight, with a mean toxicity of 449 µg STX eq./kg. PSTs were quantified in sea chervil (Alcyonidium diaphanum), the first known detection in the phylum bryozoan, as well as eleven other new vectors (>50 µg STX eq./kg), namely the opisthobranch Scaphander lignarius, the starfish Anseropoda placenta, Asterias rubens, Luidia ciliaris, Astropecten irregularis and Stichastrella rosea, the brittlestar Ophiura ophiura, the crustaceans Atelecyclus rotundatus and Munida rugosa, the sea mouse Aphrodita aculeata, and the sea urchin Psammechinus miliaris. The two species that showed consistently high PST concentrations were C. papposus and A. diaphanum. Two toxic profiles were identified, with one dominated by dcSTX (decarbamoylsaxitoxin) associated with the majority of samples across the whole sampling region. The second profile occurred only in North-Eastern England and consisted of mostly STX (Saxitoxin) and GTX2 (gonyautoxin 2). Consequently, this study highlights widespread and variable levels of PSTs in the marine benthos, together with the first evidence for toxicity in a large number of new species. These findings highlight impacts to ‘One Health’, with the unexpected sources of toxins potentially creating risks to animal, human and environmental health, with further work required to assess the severity and geographical/temporal extent of these impacts.

Benthic biodiversity on old platforms, young wind farms, and rocky reefs

The introduction of artificial hard substrates in an area dominated by a sandy seabed increases habitat available to epifouling organisms. To investigate this, samples were taken on old offshore oil and gas platforms, and data were compared with data of a young wind farm and a natural reef. Depth, sampling date, abundance of Mytilus edulis, Psammechinus miliaris, Metridium dianthus, and the presence of Tubulariidae and substrate (rock or steel) all correlated with species richness. Multivariate analysis showed a large overlap in communities on steel and rock and between the wind farm and platforms. The community changed over a gradient from deep rocks to shallow steel substrate, but no strong community differentiation was observed. Deep steel was more similar to natural rocks than shallow steel. When an artificial reef is intended to be colonized by communities similar to those on a natural reef, its structure should resemble a natural reef as much as possible.

A complete life cycle perspective on habitat requirements that control species distribution; a case study of Psammechinus miliaris in the Eastern Scheldt, Grevelingen and North Sea

The distribution and abundance of an animal is largely dependent on its habitat requirements and these requirements often change throughout its life-cycle. So to know the true impact of (a)biotic factors on a species distribution and abundance, the whole life cycle should be considered. This study aims to illustrate a fast and relatively simple approach to gain knowledge on the importance of certain habitat requirements in controlling species abundance and distribution, by comparing habitat requirements of a species’ life stages with the (a)biotic values found in nature. This is illustrated through a detailed case study of the sea urchin species, Psammechinus miliaris. Nine biotic and abiotic factors were studied, namely diet, food quantity, hydrodynamics, oxygen, hydrostatic pressure, salinity, bottom type / substrate, pH and temperature. For each of these abiotic and biotic factors, the minimum, maximum and average values were determined in the Grevelingen, North Sea and Eastern Scheldt through a literature study. The minimum and maximum habitat requirements for every abiotic and biotic factor where then determined through a literature study for each of the six P. miliaris life stages: gametes, embryo, larvae, metamorphosis, juveniles and adults. The literature search was extended by comparing data of P. miliaris observations and water temperatures in the Eastern Scheldt and Grevelingen Lake to explore the relationship between the yearly differences in water temperature and P. miliaris abundance over a 22 year period. The results show that hydrodynamics, temperature and bottom type are important limiting factors for P. miliaris, whilst hydrostatic pressure and salinity are of little to no importance. This study shows that there is a lack of research towards hydrodynamics and bottom type and it provides evidence that seawater temperatures below 1 ◦C negatively affect P. miliaris populations. This case study illustrates a relatively easy way to get a broad understanding of the habitat requirements of an organism, to identify knowledge gaps in the research area and to prioritise further research efforts.

Echinochrome A Release by Red Spherule Cells Is an Iron-Withholding Strategy of Sea Urchin Innate Immunity

Cellular immune defences in sea urchins are shared amongst the coelomocytes - a heterogeneous population of cells residing in the coelomic fluid (blood equivalent) and tissues. The most iconic coelomocyte morphotype is the red spherule cell (or amebocyte), so named due to the abundance of cytoplasmic vesicles containing the naphthoquinone pigment echinochrome A. Despite their identification over a century ago, and evidence of antiseptic properties, little progress has been made in characterising the immunocompetence of these cells. Upon exposure of red spherule cells from sea urchins, i.e., Paracentrotus lividus and Psammechinus miliaris, to microbial ligands, intact microbes, and damage signals, we observed cellular degranulation and increased detection of cell-free echinochrome in the coelomic fluid ex vivo. Treatment of the cells with ionomycin, a calcium-specific ionophore, confirmed that an increase in intracellular levels of Ca2+ is a trigger of echinochrome release. Incubating Gram-positive/negative bacteria as well as yeast with lysates of red spherule cells led to significant reductions in colony-forming units. Such antimicrobial properties were counteracted by the addition of ferric iron (Fe3+), suggesting that echinochrome acts as a primitive iron chelator in echinoid biological defences.